9Q74

Crystal structure of T. brucei EIF4E5 in complex with EIF4G1 peptide and cap-4.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 3 2 1
Unit cell lengths	140.380, 140.380, 41.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.330 - 1.570
R-factor	0.14444
Rwork	0.142
R-free	0.18258
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.810
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	121.570	1.800
High resolution limit [Å]	1.570	1.570
Number of reflections	39727	1986
<I/σ(I)>	11.3
Completeness [%]	94.4
Redundancy	7.1
CC(1/2)	0.999	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Complex at 9.4 mg/mL in 20 mM Tris-HCl, 150 mM NaCl, 2 mM DTT, pH 8.0 (pre-incubated with cap-4) with crystallization buffer containing 0.12 M monosaccharides (D-glucose, D-mannose, D-galactose, L-fucose, D-xylose, N-acetyl-D-Glucosamine), 0.1 M buffer system 1 pH 6.5 (imidazole, MES), 30% precipitant Mix2 (ethylene glycol, PEG 8000) from Morpheus Screen