9Q0M

Cryo-EM structure of PPAT-NUDT5 complex bound to adenosine-5'-monophosphate (AMP)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Amidophosphoribosyltransferase	polymer	578	63180.9	4	UniProt (Q06203)	Homo sapiens (human)	ATase,Glutamine phosphoribosylpyrophosphate amidotransferase,GPAT
2	E, F, G, H (E, F, G, H)	ADP-sugar pyrophosphatase	polymer	241	27058.3	4	UniProt (Q9UKK9)	Homo sapiens (human)	8-oxo-dGDP phosphatase,Nuclear ATP-synthesis protein NUDIX5,Nucleoside diphosphate-linked moiety X motif 5,Nudix motif 5,hNUDT5,YSA1H
3	I, K, M, O (A, B, C, D)	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
4	J, L, N, P, S... (A, B, C, D, F...)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	8	Chemie (AMP) PubChem (6083)
5	AA, BA, Q, R, U... (H, E, F, G)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG) PubChem (888)

Sequence modifications

A, B, C, D: 12 - 517 (UniProt: Q06203)

PDB	External Database	Details
Gly 518	-	expression tag
Gly 519	-	expression tag
Gly 520	-	expression tag
Lys 521	-	expression tag
Pro 522	-	expression tag
Ile 523	-	expression tag
Pro 524	-	expression tag
Asn 525	-	expression tag
Pro 526	-	expression tag
Leu 527	-	expression tag
Leu 528	-	expression tag
Gly 529	-	expression tag
Leu 530	-	expression tag
Asp 531	-	expression tag
Ser 532	-	expression tag
Thr 533	-	expression tag
Ser 534	-	expression tag
Ala 535	-	expression tag
Trp 536	-	expression tag
Ser 537	-	expression tag
His 538	-	expression tag
Pro 539	-	expression tag
Gln 540	-	expression tag
Phe 541	-	expression tag
Glu 542	-	expression tag
Lys 543	-	expression tag
Gly 544	-	expression tag
Gly 545	-	expression tag
Gly 546	-	expression tag
Ser 547	-	expression tag
Gly 548	-	expression tag
Gly 549	-	expression tag
Gly 550	-	expression tag
Ser 551	-	expression tag
Gly 552	-	expression tag
Gly 553	-	expression tag
Gly 554	-	expression tag
Ala 555	-	expression tag
Trp 556	-	expression tag
Ser 557	-	expression tag
His 558	-	expression tag
Pro 559	-	expression tag
Gln 560	-	expression tag
Phe 561	-	expression tag
Glu 562	-	expression tag
Lys 563	-	expression tag
Asn 564	-	expression tag
Gly 565	-	expression tag
Ser 566	-	expression tag
Thr 567	-	expression tag
Ser 568	-	expression tag
Gly 569	-	expression tag
Ser 570	-	expression tag
Gly 571	-	expression tag
Ala 572	-	expression tag
Thr 573	-	expression tag
Asn 574	-	expression tag
Phe 575	-	expression tag
Ser 576	-	expression tag
Leu 577	-	expression tag
Leu 578	-	expression tag
Lys 579	-	expression tag
Gln 580	-	expression tag
Ala 581	-	expression tag
Gly 582	-	expression tag
Asp 583	-	expression tag
Val 584	-	expression tag
Glu 585	-	expression tag
Glu 586	-	expression tag
Asn 587	-	expression tag
Pro 588	-	expression tag
Gly 589	-	expression tag

E, F, G, H: 2 - 219 (UniProt: Q9UKK9)

PDB	External Database	Details
Met -21	-	expression tag
Asp -20	-	expression tag
Tyr -19	-	expression tag
Lys -18	-	expression tag
Asp -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Gly -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Lys -11	-	expression tag
Asp -10	-	expression tag
His -9	-	expression tag
Asp -8	-	expression tag
Ile -7	-	expression tag
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag
Phe 74	Tyr 74	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	360956.8
Non-Polymers*	Number of molecules	20
	Total formula weight	4378.8
All*	Total formula weight	365335.5

*Water molecules are not included.