9PU5
Cryo-EM structure of human MOR-Gi1 complex protein bound with DAMGO and BMS986187
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, F (A, B) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 2 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B, G (D, C) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 342 | 37561.1 | 2 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C, H (G, E) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 80 | 8506.8 | 2 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D, I (R, F) | Mu-type opioid receptor | polymer | 388 | 43577.8 | 2 | UniProt (P35372) Pfam (PF00001) | Homo sapiens (human) | M-OR-1,MOR-1,Mu opiate receptor,Mu opioid receptor,MOP,hMOP |
| 5 | E, J (S, H) | DAMGO | polymer | 5 | 513.6 | 2 | BIRD (PRD_002308) | Homo sapiens | |
| 6 | K, L, M, O, P... (R, F) | CHOLESTEROL | non-polymer | 386.7 | 8 | Chemie (CLR) PubChem (5997) | |||
| 7 | N, T (R, F) | 3,3,6,6-tetramethyl-9-[4-[(2-methylphenyl)methoxy]phenyl]-4,5,7,9-tetrahydro-2~{H}-xanthene-1,8-dione | non-polymer | 470.6 | 2 | Chemie (A1D6B) PubChem (17379334) |
Sequence modifications
A, B: 1 - 354 (UniProt: P63096)
D, C: 2 - 340 (UniProt: P62873)
G, E: 1 - 71 (UniProt: P59768)
R, F: 1 - 388 (UniProt: P35372)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 72 | - | expression tag |
| Ser 73 | - | expression tag |
| Ala 74 | - | expression tag |
| Gly 75 | - | expression tag |
| Ser 76 | - | expression tag |
| Ala 77 | - | expression tag |
| Gly 78 | - | expression tag |
| Ser 79 | - | expression tag |
| Ala 80 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Trp 158 | Phe 158 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 261146.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 4034.4 | |
| All* | Total formula weight | 265181.0 |
