9PD4
NER dual incision complex - DuIM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | TFIIH basal transcription factor complex helicase XPB subunit | polymer | 782 | 89404.7 | 1 | UniProt (P19447) Pfam (PF13625) Pfam (PF04851) Pfam (PF16203) | Homo sapiens (human) | Basic transcription factor 2 89 kDa subunit, BTF2 p89, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH p89, Xeroderma pigmentosum group B-complementing protein |
| 2 | B (B) | General transcription and DNA repair factor IIH helicase subunit XPD | polymer | 768 | 88018.0 | 1 | UniProt (P18074) Pfam (PF06733) Pfam (PF06777) Pfam (PF13307) | Homo sapiens (human) | TFIIH subunit XPD,Basic transcription factor 2 80 kDa subunit,BTF2 p80,CXPD,DNA excision repair protein ERCC-2,DNA repair protein complementing XP-D cells,TFIIH basal transcription factor complex 80 kDa subunit,TFIIH 80 kDa subunit,TFIIH p80,Xeroderma pigmentosum group D-complementing protein |
| 3 | C (C) | General transcription factor IIH subunit 1 | polymer | 548 | 62116.5 | 1 | UniProt (P32780) Pfam (PF08567) Pfam (PF03909) | Homo sapiens (human) | Basic transcription factor 2 62 kDa subunit,BTF2 p62,General transcription factor IIH polypeptide 1,TFIIH basal transcription factor complex p62 subunit |
| 4 | D (D) | General transcription factor IIH subunit 4, p52 | polymer | 462 | 52245.2 | 1 | UniProt (Q92759) Pfam (PF18307) Pfam (PF03849) | Homo sapiens (human) | Basic transcription factor 2 52 kDa subunit, BTF2 p52, General transcription factor IIH polypeptide 4, TFIIH basal transcription factor complex p52 subunit |
| 5 | E (E) | General transcription factor IIH subunit 2 | polymer | 395 | 44482.0 | 1 | UniProt (Q13888) Pfam (PF04056) Pfam (PF07975) | Homo sapiens (human) | Basic transcription factor 2 44 kDa subunit, BTF2 p44, General transcription factor IIH polypeptide 2, TFIIH basal transcription factor complex p44 subunit |
| 6 | F (F) | General transcription factor IIH subunit 3 | polymer | 308 | 34416.0 | 1 | UniProt (Q13889) Pfam (PF03850) | Homo sapiens (human) | p34, Basic transcription factor 2 44 kDa subunit, BTF2 p44, General transcription factor IIH polypeptide 2, TFIIH basal transcription factor complex p44 subunit |
| 7 | G (G) | General transcription factor IIH subunit 5 | polymer | 71 | 8060.4 | 1 | UniProt (Q6ZYL4) Pfam (PF06331) | Homo sapiens (human) | General transcription factor IIH polypeptide 5, TFB5 ortholog, TFIIH basal transcription factor complex TTD-A subunit |
| 8 | H (S) | DNA excision repair protein ERCC-5 | polymer | 1186 | 133489.6 | 1 | UniProt (P28715) Pfam (PF00752) Pfam (PF00867) | Homo sapiens (human) | DNA repair protein complementing XP-G cells,XPG,Xeroderma pigmentosum group G-complementing protein |
| 9 | I (Q) | DNA repair endonuclease XPF | polymer | 916 | 104694.2 | 1 | UniProt (Q92889) Pfam (PF02732) | Homo sapiens (human) | DNA excision repair protein ERCC-4,DNA repair protein complementing XP-F cells,Xeroderma pigmentosum group F-complementing protein |
| 10 | J (R) | DNA excision repair protein ERCC-1 | polymer | 295 | 32402.1 | 1 | UniProt (P07992) Pfam (PF03834) Pfam (PF00633) | Homo sapiens (human) | |
| 11 | K (K) | DNA repair protein complementing XP-A cells | polymer | 273 | 31422.1 | 1 | UniProt (P23025) Pfam (PF01286) Pfam (PF05181) | Homo sapiens (human) | Xeroderma pigmentosum group A-complementing protein |
| 12 | L (L) | DNA (Cy5) | polymer | 93 | 28705.6 | 1 | synthetic construct | ||
| 13 | M (M) | DNA | polymer | 94 | 29180.6 | 1 | synthetic construct | ||
| 14 | N (N) | Replication protein A 70 kDa DNA-binding subunit | polymer | 616 | 68213.0 | 1 | UniProt (P27694) Pfam (PF04057) Pfam (PF01336) Pfam (PF16900) Pfam (PF08646) | Homo sapiens (human) | RP-A p70,Replication factor A protein 1,RF-A protein 1,Single-stranded DNA-binding protein |
| 15 | O (O) | Replication protein A 32 kDa subunit | polymer | 270 | 29276.8 | 1 | UniProt (P15927) Pfam (PF08784) | Homo sapiens (human) | RP-A p32,Replication factor A protein 2,RF-A protein 2,Replication protein A 34 kDa subunit,RP-A p34 |
| 16 | P (P) | Replication protein A 14 kDa subunit | polymer | 121 | 13583.7 | 1 | UniProt (P35244) Pfam (PF08661) | Homo sapiens (human) | RP-A p14,Replication factor A protein 3,RF-A protein 3 |
| 17 | Q (B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 18 | R, S, T, U, V... (E, F, K, N) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) |
Sequence modifications
B: 1 - 760 (UniProt: P18074)
S: 1 - 1186 (UniProt: P28715)
Q: 1 - 916 (UniProt: Q92889)
| PDB | External Database | Details |
|---|---|---|
| Asp 761 | - | expression tag |
| Tyr 762 | - | expression tag |
| Lys 763 | - | expression tag |
| Asp 764 | - | expression tag |
| Asp 765 | - | expression tag |
| Asp 766 | - | expression tag |
| Asp 767 | - | expression tag |
| Lys 768 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 812 | Asp 812 | engineered mutation |
| Arg 1053 | Gly 1053 | variant |
| Arg 1080 | Gly 1080 | variant |
| PDB | External Database | Details |
|---|---|---|
| Asp 703 | Gly 703 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 849710.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 809.5 | |
| All* | Total formula weight | 850519.9 |
