9P4E
Crystal Structure of Engineered glutamine binding protein and a Gd-DOTA ligand - no GLN bound
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Amino acid ABC transporter substrate-binding protein | polymer | 229 | 25254.7 | 1 | UniProt (C3THM2) | Escherichia coli | Glutamine ABC transporter substrate-binding protein GlnH,Glutamine ABC transporter substrate-bindnig protein,Glutamine ABC transporter,periplasmic glutamine-binding protein (TC 3.A.1.3.2),substrate-binding protein,Glutamine-binding periplasmic protein,Periplasmic glutamine-binding protein,Periplasmic glutamine-binding protein permease,Permease of periplasmic glutamine-binding protein |
| 2 | B, C, N, O (C) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 3 | D, F, G (C) | ACETATE ION | non-polymer | 59.0 | 3 | Chemie (ACT) | |||
| 4 | E (C) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | H (C) | 4-oxo-4-[(2-{2-[4,7,10-tris(carboxymethyl)-1,4,7,10-tetraazacyclododecan-1-yl]acetamido}ethyl)amino]butanoic acid | non-polymer | 546.6 | 1 | Chemie (A1C21) | |||
| 6 | I, J, K, L, M (C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 7 | P (C) | GADOLINIUM ION | non-polymer | 157.3 | 1 | Chemie (GD3) | |||
| 8 | Q (C) | water | water | 18.0 | 269 | Chemie (HOH) |
Sequence modifications
C: 1 - 226 (UniProt: C3THM2)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ile 23 | Lys 45 | engineered mutation |
| Cys 72 | Thr 94 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 25254.7 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 1667.6 | |
| All* | Total formula weight | 26922.3 |
