9OM6

22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), 4:2:2 alphaSNAP-syntaxin-1a-SNAP-25 subcomplex local refinement, hydrolyzing, class 23

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Syntaxin-1A	polymer	267	31045.9	2	UniProt (P32851) Pfam (PF00804) Pfam (PF05739)	Rattus norvegicus (Norway rat)	Neuron-specific antigen HPC-1,Synaptotagmin-associated 35 kDa protein,P35A
2	C, D (C, D)	Synaptosomal-associated protein 25	polymer	222	25036.7	2	UniProt (P60881) Pfam (PF00835)	Rattus norvegicus (Norway rat)	SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein
3	E, F, G, H (E, F, G, H)	Alpha-soluble NSF attachment protein	polymer	296	33290.7	4	UniProt (P54921)	Rattus norvegicus (Norway rat)	SNAP-alpha,N-ethylmaleimide-sensitive factor attachment protein alpha
4	I, J, K, L, M... (B, C, D, E, F...)	water	water		18.0	63	Chemie (HOH)

Sequence modifications

C, D: 1 - 206 (UniProt: P60881)

PDB	External Database	Details
Met -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Gln -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag
Ala 85	Cys 85	conflict
Ala 88	Cys 88	conflict
Ala 90	Cys 90	conflict
Ala 92	Cys 92	conflict

E, F, G, H: 1 - 295 (UniProt: P54921)

PDB	External Database	Details
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	245328.2
All*	Total formula weight	245328.2

*Water molecules are not included.