9OM5

Composite map of six VRC35 Fabs and three MEDI8852 Fabs bound to influenza H3N2 Victoria 2011 hemagglutinin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, C, E)	Hemagglutinin	polymer	348	38937.2	3	UniProt (A0A5P1MU07)	Influenza A virus
2	B, D, F (B, D, F)	Hemagglutinin	polymer	223	25410.3	3	UniProt (A0A2P1E3C0)	Influenza A virus
3	G, I, K (G, I, K)	MEDI8852 Fab heavy chain	polymer	227	24413.2	3		Homo sapiens
4	H, J, L (H, J, L)	MEDI8852 Fab light chain	polymer	206	22450.8	3		Homo sapiens
5	M, O, Q, S, U... (a, c, e, g, i...)	VRC35 Fab heavy chain	polymer	222	23909.1	6		Homo sapiens
6	N, P, R, T, V... (b, d, f, h, j...)	VRC35 Fab light chain	polymer	219	24033.9	6		Homo sapiens
7	BA, DA, FA, GA, IA... (P, R, T, U, W...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	12	In PDB GlyTouCan (G15407YE)
8	AA, CA, EA, JA, LA... (O, Q, S, X, Z...)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	7	In PDB GlyTouCan (G22768VO)
9	HA, OA (V, o)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G81315DD)
10	TA, UA, VA (B, D, F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG) PubChem (24139)
11	WA, XA (e, i)	alpha-D-mannopyranose	non-polymer		180.2	2	Chemie (MAN) PubChem (185698)

Sequence modifications

A, C, E: -15 - 327 (UniProt: A0A5P1MU07)

PDB	External Database	Details
His -7	Tyr 9	conflict
Cys 30	Thr 46	conflict
Arg 328	-	expression tag
Arg 329	-	expression tag
Arg 330	-	expression tag
Arg 331	-	expression tag
Arg 332	-	expression tag

B, D, F: 0 - 176 (UniProt: A0A2P1E3C0)

PDB	External Database	Details
Cys 47	Gln 376	conflict
Ser 177	-	expression tag
Gly 178	-	expression tag
Leu 179	-	expression tag
Glu 180	-	expression tag
Val 181	-	expression tag
Leu 182	-	expression tag
Phe 183	-	expression tag
Gln 184	-	expression tag
Gly 185	-	expression tag
Pro 186	-	expression tag
Gly 187	-	expression tag
Ser 188	-	expression tag
Gly 189	-	expression tag
Tyr 190	-	expression tag
Ile 191	-	expression tag
Pro 192	-	expression tag
Glu 193	-	expression tag
Ala 194	-	expression tag
Pro 195	-	expression tag
Arg 196	-	expression tag
Asp 197	-	expression tag
Gly 198	-	expression tag
Gln 199	-	expression tag
Ala 200	-	expression tag
Tyr 201	-	expression tag
Val 202	-	expression tag
Arg 203	-	expression tag
Lys 204	-	expression tag
Asp 205	-	expression tag
Gly 206	-	expression tag
Glu 207	-	expression tag
Trp 208	-	expression tag
Val 209	-	expression tag
Leu 210	-	expression tag
Leu 211	-	expression tag
Ser 212	-	expression tag
Thr 213	-	expression tag
Phe 214	-	expression tag
Leu 215	-	expression tag
Gly 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	621292.1
Branched	Number of molecules	21
	Total formula weight	14911.6
Non-Polymers*	Number of molecules	5
	Total formula weight	1023.9
All*	Total formula weight	637227.7

*Water molecules are not included.