9OF0

Cryo-EM Structure of Human HIF-2a-ARNT Complexed on 20-bp HRE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Endothelial PAS domain-containing protein 1	polymer	372	42684.5	1	UniProt (Q99814) Pfam (PF00989) Pfam (PF14598)	Homo sapiens (human)	EPAS-1,Basic-helix-loop-helix-PAS protein MOP2,Class E basic helix-loop-helix protein 73,bHLHe73,HIF-1-alpha-like factor,HLF,Hypoxia-inducible factor 2-alpha,HIF-2-alpha,HIF2-alpha,Member of PAS protein 2,PAS domain-containing protein 2
2	B (B)	Aryl hydrocarbon receptor nuclear translocator	polymer	381	43061.8	1	UniProt (P27540) Pfam (PF00010) Pfam (PF00989) Pfam (PF14598)	Homo sapiens (human)	ARNT protein,Class E basic helix-loop-helix protein 2,bHLHe2,Dioxin receptor,nuclear translocator,Hypoxia-inducible factor 1-beta,HIF-1-beta,HIF1-beta
3	C (C)	20-nt Hypoxia Response Element DNA (Forward)	polymer	21	6527.2	1		Homo sapiens
4	D (D)	20-nt Hypoxia Response Element DNA (Reverse)	polymer	21	6363.1	1		Homo sapiens

Sequence modifications

A: 5 - 361 (UniProt: Q99814)

PDB	External Database	Details
Met -10	-	initiating methionine
Gly -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag
Asp 2	-	expression tag
Pro 3	-	expression tag
Gly 4	-	expression tag

B: 91 - 470 (UniProt: P27540)

PDB	External Database	Details
Met 90	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	98636.6
All*	Total formula weight	98636.6

*Water molecules are not included.