9O4K

Cryo-EM Structure of the Arabidopsis GA3-GID1A-RGA-SLY1-ASK1 Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DELLA protein RGA	polymer	597	65253.3	1	UniProt (Q9SLH3) Pfam (PF12041) Pfam (PF03514)	Arabidopsis thaliana (thale cress)	GAI-related sequence,GRAS family protein 10,AtGRAS-10,Repressor on the ga1-3 mutant,Restoration of growth on ammonia protein 1
2	B (B)	Gibberellin receptor GID1A	polymer	355	39775.9	1	UniProt (Q9MAA7) Pfam (PF07859)	Arabidopsis thaliana (thale cress)	AtCXE10,Carboxylesterase 10,GID1-like protein 1,Protein GA INSENSITIVE DWARF 1A,AtGID1A
3	C (C)	F-box protein GID2	polymer	179	21070.0	1	UniProt (Q9STX3) Pfam (PF00646)	Arabidopsis thaliana (thale cress)	Protein SLEEPY 1
4	D (D)	SKP1-like protein 1A	polymer	187	21273.6	1	UniProt (Q39255) Pfam (PF03931) Pfam (PF01466)	Arabidopsis thaliana (thale cress)	SKP1-like 1,UFO-binding protein 1
5	E (B)	GIBBERELLIN A3	non-polymer		346.4	1	Chemie (GA3)

Sequence modifications

A: 1 - 587 (UniProt: Q9SLH3)

PDB	External Database	Details
Gln 163	Lys 163	engineered mutation
Gln 164	Arg 164	engineered mutation
Gln 166	Lys 166	engineered mutation
Gly 588	-	expression tag
Gly 589	-	expression tag
Trp 590	-	expression tag
Ser 591	-	expression tag
His 592	-	expression tag
Pro 593	-	expression tag
Gln 594	-	expression tag
Phe 595	-	expression tag
Glu 596	-	expression tag
Arg 597	-	expression tag

B: 1 - 345 (UniProt: Q9MAA7)

PDB	External Database	Details
Gly 346	-	expression tag
Gly 347	-	expression tag
Asp 348	-	expression tag
Tyr 349	-	expression tag
Lys 350	-	expression tag
Asp 351	-	expression tag
Asp 352	-	expression tag
Asp 353	-	expression tag
Asp 354	-	expression tag
Lys 355	-	expression tag

C: 1 - 151 (UniProt: Q9STX3)

PDB	External Database	Details
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Lys 138	Glu 138	engineered mutation
Gly 152	-	expression tag
Gly 153	-	expression tag
Trp 154	-	expression tag
Ser 155	-	expression tag
His 156	-	expression tag
Pro 157	-	expression tag
Gln 158	-	expression tag
Phe 159	-	expression tag
Glu 160	-	expression tag
Arg 161	-	expression tag

D: 1 - 160 (UniProt: Q39255)

PDB	External Database	Details
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 161	-	expression tag
Gly 162	-	expression tag
Tyr 163	-	expression tag
Lys 164	-	expression tag
Asp 165	-	expression tag
Asp 166	-	expression tag
Asp 167	-	expression tag
Asp 168	-	expression tag
Lys 169	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	147372.7
Non-Polymers*	Number of molecules	1
	Total formula weight	346.4
All*	Total formula weight	147719.1

*Water molecules are not included.