9NZ2

Cryo-EM structure of antibody 22F5 in complex with pre-fusion stabilized LayV-F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Fusion glycoprotein F0	polymer	563	61504.9	3	UniProt (A0AAX3C923)	Langya virus
2	D, F, I (D, F, I)	Antibody 22F5 Heavy Chain	polymer	216	23196.0	3		Mus musculus
3	E, G, H (E, G, H)	Antibody 22F5 Kappa Light Chain	polymer	219	24323.9	3		Mus musculus
4	J, K, L (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG) PubChem (24139)

Sequence modifications

A, B, C: 1 - 483 (UniProt: A0AAX3C923)

PDB	External Database	Details
Cys 99	Gly 99	engineered mutation
Cys 109	Ile 109	engineered mutation
Gly 484	-	expression tag
Ser 485	-	expression tag
Gly 486	-	expression tag
Tyr 487	-	expression tag
Ile 488	-	expression tag
Pro 489	-	expression tag
Glu 490	-	expression tag
Ala 491	-	expression tag
Pro 492	-	expression tag
Arg 493	-	expression tag
Asp 494	-	expression tag
Gly 495	-	expression tag
Gln 496	-	expression tag
Ala 497	-	expression tag
Tyr 498	-	expression tag
Val 499	-	expression tag
Arg 500	-	expression tag
Lys 501	-	expression tag
Asp 502	-	expression tag
Gly 503	-	expression tag
Glu 504	-	expression tag
Trp 505	-	expression tag
Val 506	-	expression tag
Leu 507	-	expression tag
Leu 508	-	expression tag
Ser 509	-	expression tag
Thr 510	-	expression tag
Phe 511	-	expression tag
Leu 512	-	expression tag
Gly 513	-	expression tag
Arg 514	-	expression tag
Ser 515	-	expression tag
Leu 516	-	expression tag
Glu 517	-	expression tag
Val 518	-	expression tag
Leu 519	-	expression tag
Phe 520	-	expression tag
Gln 521	-	expression tag
Gly 522	-	expression tag
Pro 523	-	expression tag
Gly 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag
His 532	-	expression tag
Ser 533	-	expression tag
Ala 534	-	expression tag
Trp 535	-	expression tag
Ser 536	-	expression tag
His 537	-	expression tag
Pro 538	-	expression tag
Gln 539	-	expression tag
Phe 540	-	expression tag
Glu 541	-	expression tag
Lys 542	-	expression tag
Gly 543	-	expression tag
Gly 544	-	expression tag
Gly 545	-	expression tag
Ser 546	-	expression tag
Gly 547	-	expression tag
Gly 548	-	expression tag
Gly 549	-	expression tag
Gly 550	-	expression tag
Ser 551	-	expression tag
Gly 552	-	expression tag
Gly 553	-	expression tag
Ser 554	-	expression tag
Ala 555	-	expression tag
Trp 556	-	expression tag
Ser 557	-	expression tag
His 558	-	expression tag
Pro 559	-	expression tag
Gln 560	-	expression tag
Phe 561	-	expression tag
Glu 562	-	expression tag
Lys 563	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	327074.5
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	327738.1

*Water molecules are not included.