9NYB

Crystal structure of the pre-reactive state of porcine OAS1 in complex with dsRNA, two ApCpp substrate analogs, three catalytic Mn2+ ions.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	2'-5'-oligoadenylate synthase 1	polymer	357	41278.3	1	UniProt (Q29599)	Sus scrofa (pig)	(2-5')oligo(A) synthase 1,2-5A synthase 1,p42 OAS
2	B (B)	RNA (5'-R(*GP*GP*CP*UP*UP*UP*UP*GP*AP*CP*CP*UP*UP*UP*AP*UP*GP*AP*A)-3')	polymer	19	6017.6	1		synthetic construct
3	C (C)	RNA (5'-R(*UP*UP*CP*AP*UP*AP*AP*AP*GP*GP*UP*CP*AP*AP*AP*AP*GP*CP*C)-3')	polymer	19	6069.7	1		synthetic construct
4	D, E (A)	DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER	non-polymer		505.2	2	Chemie (APC)
5	F, G, H, I, K... (A, B, C)	MANGANESE (II) ION	non-polymer		54.9	6	Chemie (MN) PubChem (27854)
6	J (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	M, N, O (A, B, C)	water	water		18.0	874	Chemie (HOH)

Sequence modifications

A: 1 - 349 (UniProt: Q29599)

PDB	External Database	Details
Gly 350	-	expression tag
Ser 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	53365.6
Non-Polymers*	Number of molecules	9
	Total formula weight	1402.1
All*	Total formula weight	54767.7

*Water molecules are not included.