9NYB
Crystal structure of the pre-reactive state of porcine OAS1 in complex with dsRNA, two ApCpp substrate analogs, three catalytic Mn2+ ions.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-14 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.030, 72.030, 206.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.712 - 1.600 |
| Rwork | 0.152 |
| R-free | 0.21140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.040 |
| RMSD bond angle | 2.033 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.712 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.056 | 0.803 |
| Number of reflections | 72726 | 11850 |
| <I/σ(I)> | 23.42 | 2.15 |
| Completeness [%] | 99.9 | |
| Redundancy | 26.38 | |
| CC(1/2) | 1.000 | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 100 mM Tris HCl pH 7.6, 33% (v / v) PEG200 |
