9NXS

Crystal structure of the post-reactive state of porcine OAS1 in complex with dsRNA and products 25A2 and PPi bound to the catalytic center.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	2'-5'-oligoadenylate synthase 1	polymer	357	41278.3	1	UniProt (Q29599)	Sus scrofa (pig)	(2-5')oligo(A) synthase 1,2-5A synthase 1,p42 OAS
2	B (B)	RNA (5'-R(*GP*GP*CP*UP*UP*UP*UP*GP*AP*CP*CP*UP*UP*UP*AP*UP*GP*AP*A)-3')	polymer	19	6017.6	1		synthetic construct
3	C (C)	RNA (5'-R(*UP*UP*CP*AP*UP*AP*AP*AP*GP*GP*UP*CP*AP*AP*AP*AP*GP*CP*C)-3')	polymer	19	6069.7	1		synthetic construct
4	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
5	E (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN) PubChem (27854)
6	F (A)	[[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-4-[[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-4-[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-3-hydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-3-hydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl] phosphono hydrogen phosphate	non-polymer		1165.6	1	Chemie (25L) PubChem (125229) PubChem (433443) PubChem (503593)
7	G (A)	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP) PubChem (4995)
8	H (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
9	I, J, K (A, B, C)	water	water		18.0	642	Chemie (HOH)

Sequence modifications

A: 1 - 349 (UniProt: Q29599)

PDB	External Database	Details
Gly 350	-	expression tag
Ser 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	53365.6
Non-Polymers*	Number of molecules	5
	Total formula weight	1482.9
All*	Total formula weight	54848.4

*Water molecules are not included.