9NU8

Structure of MurJ in complex with single gene lysis protein from phage Changjiang3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Lipid II flippase MurJ	polymer	614	66668.7	1	UniProt (P0AF16)	Escherichia coli K-12	Peptidoglycan biosynthesis protein MurJ
2	B (B)	Lysis protein	polymer	70	7324.4	1		Changjiang levi-like virus 3

Sequence modifications

A: 3 - 500 (UniProt: P0AF16)

PDB	External Database	Details
Met -103	-	initiating methionine
Ala -102	-	expression tag
Asp -101	-	expression tag
Leu -100	-	expression tag
Glu -99	-	expression tag
Asp -98	-	expression tag
Asn -97	-	expression tag
Trp -96	-	expression tag
Glu -95	-	expression tag
Thr -94	-	expression tag
Leu -93	-	expression tag
Asn -92	-	expression tag
Asp -91	-	expression tag
Asn -90	-	expression tag
Leu -89	-	expression tag
Lys -88	-	expression tag
Val -87	-	expression tag
Ile -86	-	expression tag
Glu -85	-	expression tag
Lys -84	-	expression tag
Ala -83	-	expression tag
Asp -82	-	expression tag
Asn -81	-	expression tag
Ala -80	-	expression tag
Ala -79	-	expression tag
Gln -78	-	expression tag
Val -77	-	expression tag
Lys -76	-	expression tag
Asp -75	-	expression tag
Ala -74	-	expression tag
Leu -73	-	expression tag
Thr -72	-	expression tag
Lys -71	-	expression tag
Met -70	-	expression tag
Arg -69	-	expression tag
Ala -68	-	expression tag
Ala -67	-	expression tag
Ala -66	-	expression tag
Leu -65	-	expression tag
Asp -64	-	expression tag
Ala -63	-	expression tag
Gln -62	-	expression tag
Lys -61	-	expression tag
Ala -60	-	expression tag
Thr -59	-	expression tag
Pro -58	-	expression tag
Pro -57	-	expression tag
Lys -56	-	expression tag
Leu -55	-	expression tag
Glu -54	-	expression tag
Asp -53	-	expression tag
Lys -52	-	expression tag
Ser -51	-	expression tag
Pro -50	-	expression tag
Asp -49	-	expression tag
Ser -48	-	expression tag
Pro -47	-	expression tag
Glu -46	-	expression tag
Met -45	-	expression tag
Lys -44	-	expression tag
Asp -43	-	expression tag
Phe -42	-	expression tag
Arg -41	-	expression tag
His -40	-	expression tag
Gly -39	-	expression tag
Phe -38	-	expression tag
Asp -37	-	expression tag
Ile -36	-	expression tag
Leu -35	-	expression tag
Val -34	-	expression tag
Gly -33	-	expression tag
Gln -32	-	expression tag
Ile -31	-	expression tag
Asp -30	-	expression tag
Asp -29	-	expression tag
Ala -28	-	expression tag
Leu -27	-	expression tag
Lys -26	-	expression tag
Leu -25	-	expression tag
Ala -24	-	expression tag
Asn -23	-	expression tag
Glu -22	-	expression tag
Gly -21	-	expression tag
Lys -20	-	expression tag
Val -19	-	expression tag
Lys -18	-	expression tag
Glu -17	-	expression tag
Ala -16	-	expression tag
Gln -15	-	expression tag
Ala -14	-	expression tag
Ala -13	-	expression tag
Ala -12	-	expression tag
Glu -11	-	expression tag
Gln -10	-	expression tag
Leu -9	-	expression tag
Lys -8	-	expression tag
Thr -7	-	expression tag
Thr -6	-	expression tag
Arg -5	-	expression tag
Asn -4	-	expression tag
Ala -3	-	expression tag
Tyr -2	-	expression tag
Ile -1	-	expression tag
Gln 0	-	expression tag
Lys 1	-	expression tag
Tyr 2	-	expression tag
Phe 5	Lys 5	engineered mutation
Ile 12	Ser 12	engineered mutation
Ala 13	Met 13	engineered mutation
Ser 501	-	expression tag
Ala 502	-	expression tag
Trp 503	-	expression tag
Ser 504	-	expression tag
His 505	-	expression tag
Pro 506	-	expression tag
Gln 507	-	expression tag
Phe 508	-	expression tag
Glu 509	-	expression tag
Lys 510	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73993.1
All*	Total formula weight	73993.1

*Water molecules are not included.