9NU4

Structure of MurJ in complex with single gene lysis protein from phage M

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Lipid II flippase MurJ	polymer	614	66668.7	1	UniProt (P0AF16)	Escherichia coli K-12	Peptidoglycan biosynthesis protein MurJ
2	B (B)	Lysis protein	polymer	66	6625.3	1	UniProt (K7QK87)	Enterobacteria phage M

Sequence modifications

A: 3 - 500 (UniProt: P0AF16)

PDB	External Database	Details
Met -103	-	initiating methionine
Ala -102	-	expression tag
Asp -101	-	expression tag
Leu -100	-	expression tag
Glu -99	-	expression tag
Asp -98	-	expression tag
Asn -97	-	expression tag
Trp -96	-	expression tag
Glu -95	-	expression tag
Thr -94	-	expression tag
Leu -93	-	expression tag
Asn -92	-	expression tag
Asp -91	-	expression tag
Asn -90	-	expression tag
Leu -89	-	expression tag
Lys -88	-	expression tag
Val -87	-	expression tag
Ile -86	-	expression tag
Glu -85	-	expression tag
Lys -84	-	expression tag
Ala -83	-	expression tag
Asp -82	-	expression tag
Asn -81	-	expression tag
Ala -80	-	expression tag
Ala -79	-	expression tag
Gln -78	-	expression tag
Val -77	-	expression tag
Lys -76	-	expression tag
Asp -75	-	expression tag
Ala -74	-	expression tag
Leu -73	-	expression tag
Thr -72	-	expression tag
Lys -71	-	expression tag
Met -70	-	expression tag
Arg -69	-	expression tag
Ala -68	-	expression tag
Ala -67	-	expression tag
Ala -66	-	expression tag
Leu -65	-	expression tag
Asp -64	-	expression tag
Ala -63	-	expression tag
Gln -62	-	expression tag
Lys -61	-	expression tag
Ala -60	-	expression tag
Thr -59	-	expression tag
Pro -58	-	expression tag
Pro -57	-	expression tag
Lys -56	-	expression tag
Leu -55	-	expression tag
Glu -54	-	expression tag
Asp -53	-	expression tag
Lys -52	-	expression tag
Ser -51	-	expression tag
Pro -50	-	expression tag
Asp -49	-	expression tag
Ser -48	-	expression tag
Pro -47	-	expression tag
Glu -46	-	expression tag
Met -45	-	expression tag
Lys -44	-	expression tag
Asp -43	-	expression tag
Phe -42	-	expression tag
Arg -41	-	expression tag
His -40	-	expression tag
Gly -39	-	expression tag
Phe -38	-	expression tag
Asp -37	-	expression tag
Ile -36	-	expression tag
Leu -35	-	expression tag
Val -34	-	expression tag
Gly -33	-	expression tag
Gln -32	-	expression tag
Ile -31	-	expression tag
Asp -30	-	expression tag
Asp -29	-	expression tag
Ala -28	-	expression tag
Leu -27	-	expression tag
Lys -26	-	expression tag
Leu -25	-	expression tag
Ala -24	-	expression tag
Asn -23	-	expression tag
Glu -22	-	expression tag
Gly -21	-	expression tag
Lys -20	-	expression tag
Val -19	-	expression tag
Lys -18	-	expression tag
Glu -17	-	expression tag
Ala -16	-	expression tag
Gln -15	-	expression tag
Ala -14	-	expression tag
Ala -13	-	expression tag
Ala -12	-	expression tag
Glu -11	-	expression tag
Gln -10	-	expression tag
Leu -9	-	expression tag
Lys -8	-	expression tag
Thr -7	-	expression tag
Thr -6	-	expression tag
Arg -5	-	expression tag
Asn -4	-	expression tag
Ala -3	-	expression tag
Tyr -2	-	expression tag
Ile -1	-	expression tag
Gln 0	-	expression tag
Lys 1	-	expression tag
Tyr 2	-	expression tag
Phe 5	Lys 5	engineered mutation
Ile 12	Ser 12	engineered mutation
Ala 13	Met 13	engineered mutation
Ser 501	-	expression tag
Ala 502	-	expression tag
Trp 503	-	expression tag
Ser 504	-	expression tag
His 505	-	expression tag
Pro 506	-	expression tag
Gln 507	-	expression tag
Phe 508	-	expression tag
Glu 509	-	expression tag
Lys 510	-	expression tag

B: 2 - 37 (UniProt: K7QK87)

PDB	External Database	Details
Met -28	-	initiating methionine
Gly -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73294.0
All*	Total formula weight	73294.0

*Water molecules are not included.