9NQ7

Cryo-EM structure of Csm/AcrIIIA2/enolase 4:3 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	RNA (41-MER)	polymer	41	13055.8	1		Streptococcus thermophilus
2	B (H)	CRISPR system Cms protein Csm5	polymer	357	41226.3	1	UniProt (A0A0A7HF79)	Streptococcus thermophilus	CRISPR type III A-associated protein Csm5
3	C (A)	CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)	polymer	758	86930.7	1	UniProt (A0A0A7HFE1)	Streptococcus thermophilus	ssDNase Cas10,Cyclic oligoadenylate synthase,StCas10
4	D (B)	CRISPR system Cms protein Csm4	polymer	299	33829.0	1	UniProt (A0A811IGE5)	Streptococcus thermophilus
5	E, G, H, I (F, C, E, G)	CRISPR system Cms endoribonuclease Csm3	polymer	220	24585.8	4	UniProt (A0AAN1ZZJ5)	Streptococcus thermophilus	CRISPR type III A-associated RAMP protein Csm3
6	F, J, K (D, I, J)	CRISPR system Cms protein Csm2	polymer	136	16190.5	3	UniProt (A0A8D6U6W4)	Streptococcus thermophilus	CRISPR type III A-associated protein Csm2
7	L, M, N, O, P... (S, L, P, K, M...)	Enolase	polymer	434	46948.5	8	UniProt (Q03LI0)	Streptococcus thermophilus	2-phospho-D-glycerate hydro-lyase,2-phosphoglycerate dehydratase
8	T (T)	AcrIIIA2	polymer	105	12228.9	1	UniProt (A0A3G8FB66)	Streptococcus phage vB_SthS-VA214

Sequence modifications

D, I, J: 1 - 130 (UniProt: A0A8D6U6W4)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

S, L, P, K, M, O, N, Q: 1 - 434 (UniProt: Q03LI0)

PDB	External Database	Details
His 253	Arg 253	conflict
Gly 257	Asp 257	conflict

T: 1 - 104 (UniProt: A0A3G8FB66)

PDB	External Database	Details
Val 105	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	709773.4
All*	Total formula weight	709773.4

*Water molecules are not included.