9NPU
Pre-catalytic ternary complex of DNA Polymerase Lambda with bound 1-nt gapped SSB substrate containing template ribonucleotide opposite primer terminus, and incoming dUMPNPP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase lambda | polymer | 346 | 38551.0 | 1 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (human) | Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa |
| 2 | B (T) | DNA/RNA (5'-D(*CP*GP*GP*CP*A)-R(P*G)-D(P*TP*AP*CP*TP*G)-3') | polymer | 11 | 3390.2 | 1 | synthetic construct | ||
| 3 | C (P) | DNA (5'-D(*CP*AP*GP*TP*AP*C)-3') | polymer | 6 | 1793.2 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(P*GP*CP*CP*G)-3') | polymer | 4 | 1191.8 | 1 | synthetic construct | ||
| 5 | E, F (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 6 | G (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | H (A) | 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DUP) | |||
| 8 | I, J (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 9 | K (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 10 | L (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 11 | M, N, O, P (A, T, P, D) | water | water | 18.0 | 265 | Chemie (HOH) |
Sequence modifications
A: 234 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Gly 230 | - | expression tag |
| Ser 231 | - | expression tag |
| Ala 232 | - | expression tag |
| Ala 233 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 44926.2 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 858.4 | |
| All* | Total formula weight | 45784.6 |
