9NPU

Pre-catalytic ternary complex of DNA Polymerase Lambda with bound 1-nt gapped SSB substrate containing template ribonucleotide opposite primer terminus, and incoming dUMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2025-02-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9202
Spacegroup name	P 21 21 21
Unit cell lengths	56.204, 59.923, 140.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.990 - 1.630
R-factor	0.1792
Rwork	0.178
R-free	0.19920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.083
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.010	1.690
High resolution limit [Å]	1.630	1.630
Rmerge	0.110	1.541
Rmeas	0.116	1.631
Rpim	0.035	0.526
Number of reflections	60232	5928
<I/σ(I)>	11	1.4
Completeness [%]	100.0	100
Redundancy	10.4	9.1
CC(1/2)	0.999	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	68 mM Na cacodylate pH 6.5, 20.4% (w/v) PEG8000, 136 mM ammonium sulfate, 12% (v/v) glycerol