9NO4
Cryo-EM structure of Csm/AcrIIIA2/enolase 3:2 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | CRISPR system Cms protein Csm4 | polymer | 299 | 33829.0 | 1 | UniProt (A0A811IGE5) | Streptococcus thermophilus | |
| 2 | B, C (E, F) | CRISPR system Cms endoribonuclease Csm3 | polymer | 220 | 24585.8 | 2 | UniProt (A0AAN1ZZJ5) | Streptococcus thermophilus | CRISPR type III A-associated RAMP protein Csm3 |
| 3 | D (G) | CRISPR system Cms endoribonuclease Csm3 | polymer | 220 | 24555.9 | 1 | UniProt (A0AAN1ZZJ5) | Streptococcus thermophilus | CRISPR type III A-associated RAMP protein Csm3 |
| 4 | E (N) | crRNA RNA (35-MER) | polymer | 35 | 11118.7 | 1 | Streptococcus thermophilus | ||
| 5 | F, G (C, D) | CRISPR system Cms protein Csm2 | polymer | 130 | 15361.6 | 2 | UniProt (A0A8D6U6W4) | Streptococcus thermophilus | CRISPR type III A-associated protein Csm2 |
| 6 | H (H) | CRISPR system Cms protein Csm5 | polymer | 357 | 41226.3 | 1 | UniProt (A0A0A7HF79) | Streptococcus thermophilus | CRISPR type III A-associated protein Csm5 |
| 7 | I, J, L, M, N... (I, J, K, L, M...) | Enolase | polymer | 434 | 46948.5 | 8 | UniProt (Q03LI0) | Streptococcus thermophilus | 2-phospho-D-glycerate hydro-lyase,2-phosphoglycerate dehydratase |
| 8 | K (R) | AcrIIIA2 | polymer | 105 | 12226.9 | 1 | UniProt (A0A3G8FB66) | Streptococcus phage 2972 | |
| 9 | R (A) | CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) | polymer | 758 | 86930.7 | 1 | UniProt (A0A0A7HFE1) | Streptococcus thermophilus | ssDNase Cas10,Cyclic oligoadenylate synthase,StCas10 |
| 10 | S, T, U, V, W... (I, J, K, L, M...) | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) |
Sequence modifications
G: 1 - 220 (UniProt: A0AAN1ZZJ5)
I, J, K, L, M, O, P, Q: 1 - 434 (UniProt: Q03LI0)
| PDB | External Database | Details |
|---|---|---|
| Val 219 | Glu 219 | conflict |
| PDB | External Database | Details |
|---|---|---|
| His 253 | Arg 253 | conflict |
| Gly 257 | Asp 257 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 665370.2 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 194.4 | |
| All* | Total formula weight | 665564.6 |
