9NE1

Crystal structure of a bacterial DOT1L methyl-transferase in complex with S-adenosyl homocysteine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Histone-lysine N-methyltransferase, H3 lysine-79 specific	polymer	227	25783.7	1	UniProt (A0A3A6VYM1)	Legionella pneumophila	Histone H3-K79 methyltransferase
2	B (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
3	C (A)	water	water		18.0	44	Chemie (HOH)

Sequence modifications

A: 31 - 238 (UniProt: A0A3A6VYM1)

PDB	External Database	Details
Met 12	-	expression tag
Lys 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
Ser 20	-	expression tag
Ala 21	-	expression tag
Gly 22	-	expression tag
Leu 23	-	expression tag
Glu 24	-	expression tag
Val 25	-	expression tag
Leu 26	-	expression tag
Phe 27	-	expression tag
Gln 28	-	expression tag
Gly 29	-	expression tag
Pro 30	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	25783.7
Non-Polymers*	Number of molecules	1
	Total formula weight	384.4
All*	Total formula weight	26168.2

*Water molecules are not included.