9NB1
CRYO-EM STRUCTURE OF human U7 SNRNP WITH MUTANT LSM11 that disrupts contacts with CPSF73
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Small nuclear ribonucleoprotein Sm D3 | polymer | 146 | 16111.7 | 1 | UniProt (P62318) Pfam (PF01423) | Homo sapiens (human) | Sm-D3,snRNP core protein D3 |
| 2 | B (C) | U7 snRNA-associated Sm-like protein LSm10 | polymer | 123 | 14102.1 | 1 | UniProt (Q969L4) Pfam (PF01423) | Homo sapiens (human) | |
| 3 | C (E) | Small nuclear ribonucleoprotein E | polymer | 92 | 10817.6 | 1 | UniProt (P62304) Pfam (PF01423) | Homo sapiens (human) | snRNP-E,Sm protein E,SmE |
| 4 | D (F) | Small nuclear ribonucleoprotein F | polymer | 86 | 9734.2 | 1 | UniProt (P62306) Pfam (PF01423) | Homo sapiens (human) | snRNP-F,Sm protein F,SmF |
| 5 | E (G) | Small nuclear ribonucleoprotein G | polymer | 84 | 9579.2 | 1 | UniProt (P62308) Pfam (PF01423) | Homo sapiens (human) | snRNP-G,Sm protein G,SmG |
| 6 | F (J) | Symplekin | polymer | 1072 | 120355.1 | 1 | UniProt (Q92797) Pfam (PF11935) Pfam (PF12295) | Homo sapiens (human) | |
| 7 | G (K) | CstF77 | polymer | 8 | 1094.2 | 1 | Trichoplusia ni | ||
| 8 | H (Y) | modified H2a pre-mRNA | polymer | 52 | 16627.0 | 1 | Mus musculus | ||
| 9 | I (Z) | U7 snRNA | polymer | 60 | 19097.2 | 1 | GenBank (1633547) | Homo sapiens | |
| 10 | J (B) | Small nuclear ribonucleoprotein-associated proteins B and B' | polymer | 95 | 10911.9 | 1 | UniProt (P14678) Pfam (PF01423) | Homo sapiens (human) | snRNP-B,Sm protein B/B',SmB/B' |
| 11 | K (D) | U7 snRNA-associated Sm-like protein LSm11 | polymer | 259 | 28399.3 | 1 | Homo sapiens | ||
| 12 | L (H) | Cleavage and polyadenylation specificity factor subunit 3 | polymer | 684 | 77580.9 | 1 | UniProt (Q9UKF6) Pfam (PF11718) Pfam (PF00753) Pfam (PF10996) Pfam (PF07521) | Homo sapiens (human) | Cleavage and polyadenylation specificity factor 73 kDa subunit,CPSF 73 kDa subunit,mRNA 3'-end-processing endonuclease CPSF-73 |
| 13 | M (I) | Cleavage and polyadenylation specificity factor subunit 2 | polymer | 782 | 88597.7 | 1 | UniProt (Q9P2I0) Pfam (PF16661) Pfam (PF10996) Pfam (PF07521) Pfam (PF13299) | Homo sapiens (human) | Cleavage and polyadenylation specificity factor 100 kDa subunit,CPSF 100 kDa subunit |
| 14 | N, O (H) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A: 1 - 126 (UniProt: P62318)
G: 1 - 76 (UniProt: P62308)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Leu 77 | - | expression tag |
| Glu 78 | - | expression tag |
| His 79 | - | expression tag |
| His 80 | - | expression tag |
| His 81 | - | expression tag |
| His 82 | - | expression tag |
| His 83 | - | expression tag |
| His 84 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 423008.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 130.8 | |
| All* | Total formula weight | 423138.9 |
