9N9G

Cryo-EM structure of the human Hec1-Nuf2 dimer bound to the paclitaxel-stabilized microtubule

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I (1, N, n)	Kinetochore protein NDC80 homolog	polymer	645	74247.9	3	UniProt (O14777) Pfam (PF03801) Pfam (PF18077) Pfam (PF24487)	Homo sapiens (human)	Highly expressed in cancer protein,Kinetochore protein Hec1,HsHec1,Kinetochore-associated protein 2,Retinoblastoma-associated protein HEC
2	B, F (A, a)	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (Q2XVP4) Pfam (PF00091) Pfam (PF03953)	Sus scrofa (pig)	Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain
3	C, G (B, b)	Tubulin beta chain	polymer	445	49907.8	2	UniProt (P02554) Pfam (PF00091) Pfam (PF03953)	Sus scrofa (pig)	Beta-tubulin
4	D, H (F, f)	Kinetochore protein Nuf2	polymer	467	54619.9	2	UniProt (Q9BZD4) Pfam (PF03800)	Homo sapiens (human)	hNuf2,hNuf2R,hsNuf2,Cell division cycle-associated protein 1
5	J, O (A, a)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486)
6	K, M, P, Q (A, B, a)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG) PubChem (888)
7	L, R (B, b)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) PubChem (136073927)
8	N, S (B, b)	TAXOL	non-polymer		853.9	2	Chemie (TA1) PubChem (16757828) PubChem (16760674) PubChem (21458255) PubChem (23307219) PubChem (23412627) PubChem (23636632) PubChem (24848897) PubChem (24867246) PubChem (44323108) PubChem (44356961) PubChem (44403716) PubChem (46781469) PubChem (49868078) PubChem (54259298) PubChem (57908851) PubChem (57946364) PubChem (58803237) PubChem (59844545) PubChem (59904698) PubChem (60123443) PubChem (70943371) PubChem (71096818) PubChem (73759783) PubChem (101598423) PubChem (118528174) PubChem (124747271) PubChem (124747273) PubChem (124747274) PubChem (126761000) PubChem (133052515) PubChem (133640187) PubChem (134715514) PubChem (143890766) PubChem (152743329) PubChem (154828192) PubChem (144453087) PubChem (160585532) PubChem (163077210) PubChem (163077213) PubChem (163077214) PubChem (163077215) PubChem (165084161) PubChem (166711459) PubChem (167423928) PubChem (170014955) PubChem (170362150) PubChem (16760177) PubChem (16742549) PubChem (21733096) PubChem (23307242) PubChem (23307253) PubChem (23307913) PubChem (24754091) PubChem (24207713) PubChem (24791027) PubChem (25200067) PubChem (42613129) PubChem (42620039) PubChem (44155032) PubChem (44276363) PubChem (44279927) PubChem (44335735) PubChem (44291178) PubChem (44328863) PubChem (44342632) PubChem (44356895) PubChem (44378736) PubChem (45489039) PubChem (46782671) PubChem (71209844) PubChem (53313247) PubChem (53298534) PubChem (54259297) PubChem (54259299) PubChem (57489669) PubChem (57999380) PubChem (58460861) PubChem (58777027) PubChem (58798493) PubChem (58596717) PubChem (59048611) PubChem (59042813) PubChem (58877402) PubChem (60005248) PubChem (59873251) PubChem (59914794) PubChem (59919147) PubChem (60123436) PubChem (60011010) PubChem (60147315) PubChem (66795656) PubChem (14060298) PubChem (176586897) PubChem (177678861) PubChem (36314) PubChem (60915) PubChem (177409) PubChem (184492) PubChem (70801836) PubChem (70842048) PubChem (70848451) PubChem (70856170) PubChem (441276) PubChem (73707502) PubChem (73759704) PubChem (89493063) PubChem (89847578) PubChem (90057663) PubChem (91122402) PubChem (91885464) PubChem (5317123) PubChem (5321715) PubChem (101920282) PubChem (5352019) PubChem (121459571) PubChem (122361554) PubChem (6604028) PubChem (6604616) PubChem (6604814) PubChem (6713921) PubChem (6915727) PubChem (124747272) PubChem (124893098) PubChem (9875988) PubChem (126968948) PubChem (129395998) PubChem (129627423) PubChem (10748131) PubChem (132517454) PubChem (11320430) PubChem (11354895) PubChem (134368714) PubChem (134972678) PubChem (137705323) PubChem (138106885) PubChem (140731501) PubChem (140512017) PubChem (146034004) PubChem (153289350) PubChem (154037993) PubChem (155982214) PubChem (159121804) PubChem (160053861) PubChem (159716711) PubChem (160171678) PubChem (160585530) PubChem (163077211) PubChem (163077212) PubChem (163077209) PubChem (163284947) PubChem (4666) PubChem (168322423) PubChem (169917882) PubChem (171036874) PubChem (175758871) PubChem (5460441) PubChem (9854162) PubChem (9897470) PubChem (10033513) PubChem (10485732) PubChem (11506104) PubChem (11828953) PubChem (14060299) PubChem (14060304) PubChem (14060306) PubChem (14060309) PubChem (15983941)

Sequence modifications

1, N, n: 1 - 642 (UniProt: O14777)

PDB	External Database	Details
Ala -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

F, f: 1 - 464 (UniProt: Q9BZD4)

PDB	External Database	Details
Ala -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	532208.0
Non-Polymers*	Number of molecules	10
	Total formula weight	3737.8
All*	Total formula weight	535945.8

*Water molecules are not included.