9N9G
Cryo-EM structure of the human Hec1-Nuf2 dimer bound to the paclitaxel-stabilized microtubule
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I (1, N, n) | Kinetochore protein NDC80 homolog | polymer | 645 | 74247.9 | 3 | UniProt (O14777) | Homo sapiens (human) | Highly expressed in cancer protein,Kinetochore protein Hec1,HsHec1,Kinetochore-associated protein 2,Retinoblastoma-associated protein HEC |
| 2 | B, F (A, a) | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 2 | UniProt (Q2XVP4) | Sus scrofa (pig) | Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain |
| 3 | C, G (B, b) | Tubulin beta chain | polymer | 445 | 49907.8 | 2 | UniProt (P02554) | Sus scrofa (pig) | Beta-tubulin |
| 4 | D, H (F, f) | Kinetochore protein Nuf2 | polymer | 467 | 54619.9 | 2 | UniProt (Q9BZD4) | Homo sapiens (human) | hNuf2,hNuf2R,hsNuf2,Cell division cycle-associated protein 1 |
| 5 | J, O (A, a) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) | |||
| 6 | K, M, P, Q (A, B, a) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 7 | L, R (B, b) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 8 | N, S (B, b) | TAXOL | non-polymer | 853.9 | 2 | Chemie (TA1) |
Sequence modifications
1, N, n: 1 - 642 (UniProt: O14777)
F, f: 1 - 464 (UniProt: Q9BZD4)
| PDB | External Database | Details |
|---|---|---|
| Ala -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 532208.0 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 3737.8 | |
| All* | Total formula weight | 535945.8 |
