9N09

Human M4 muscarinic acetylcholine receptor Gi1 complex (mini-Gsi chimera) bound to the bitopic agonist C110

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	mini-Gs/i chimera	polymer	257	29057.9	1		Homo sapiens
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	349	38402.9	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (N)	Nb35	polymer	128	13885.4	1		Lama glama
5	E (R)	Muscarinic acetylcholine receptor M4	polymer	493	54947.7	1	UniProt (P08173)	Homo sapiens (human)
6	F (R)	methyl 4-[4-(3-methyl-2-oxidanylidene-benzimidazol-1-yl)piperidin-1-yl]piperidine-1-carboxylate	non-polymer		372.5	1	Chemie (5XI)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 2 - 479 (UniProt: P08173)

PDB	External Database	Details
Asp -5	-	expression tag
Tyr -4	-	expression tag
Lys -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Asp 1	-	expression tag
His 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	144023.9
Non-Polymers*	Number of molecules	1
	Total formula weight	372.5
All*	Total formula weight	144396.4

*Water molecules are not included.