9MYS
The cryo-EM structure of the mutant MHKKKQ DNMT1-umNCP complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA (cytosine-5)-methyltransferase 1 | polymer | 1250 | 140999.6 | 1 | UniProt (P26358) Pfam (PF12047) Pfam (PF02008) Pfam (PF01426) Pfam (PF00145) | Homo sapiens (human) | Dnmt1,CXXC-type zinc finger protein 9,DNA methyltransferase HsaI,DNA MTase HsaI,M.HsaI,MCMT |
| 2 | B, H (N, n) | Histone H2A | polymer | 130 | 14109.4 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 3 | C, I (O, o) | Histone H2B 1.1 | polymer | 123 | 13655.9 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 4 | D, J (P, p) | Histone H3 | polymer | 136 | 15367.0 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 5 | E, K (Q, q) | Histone H4 | polymer | 103 | 11454.5 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 6 | F (X) | Nucleosome 601 DNA (149-MER) | polymer | 149 | 46230.5 | 1 | Escherichia coli | ||
| 7 | G (Y) | Nucleosome 601 DNA (149-MER) | polymer | 149 | 45754.1 | 1 | Escherichia coli | ||
| 8 | L, M, N, O (A) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) PubChem (32051) |
Sequence modifications
A: 351 - 1600 (UniProt: P26358)
O, o: 1 - 122 (UniProt: P02281)
P, p: 0 - 135 (UniProt: A0A310TTQ1)
| PDB | External Database | Details |
|---|---|---|
| Ala 718 | Met 718 | engineered mutation |
| Ala 719 | His 719 | engineered mutation |
| Ala 723 | Lys 723 | engineered mutation |
| Ala 724 | Lys 724 | engineered mutation |
| Ala 725 | Lys 725 | engineered mutation |
| Ala 726 | Gln 726 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Thr 29 | Ser 33 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 18 | Lys 19 | conflict |
| Cys 23 | Lys 24 | conflict |
| Ser 110 | Cys 111 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 342158.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 342419.7 |
