9MHG

Cryo EM reconstruction of PI3KC3-C1 in complex with Human RAB1A(Q70L), VPS34 kinase domain in the inactive conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phosphoinositide 3-kinase regulatory subunit 4	polymer	1409	158808.5	1	UniProt (Q99570) Pfam (PF00069) Pfam (PF22956) Pfam (PF00400)	Homo sapiens (human)	PI3-kinase regulatory subunit 4,PI3-kinase p150 subunit,Phosphoinositide 3-kinase adaptor protein
2	B (B)	Phosphatidylinositol 3-kinase catalytic subunit type 3	polymer	887	101680.3	1	UniProt (Q8NEB9) Pfam (PF00792) Pfam (PF00613) Pfam (PF00454)	Homo sapiens (human)	PI3-kinase type 3,PI3K type 3,PtdIns-3-kinase type 3,Phosphatidylinositol 3-kinase p100 subunit,Phosphoinositide-3-kinase class 3,hVps34
3	C (C)	Beclin 1-associated autophagy-related key regulator	polymer	492	55387.3	1	UniProt (Q6ZNE5) Pfam (PF10186)	Homo sapiens (human)
4	D (D)	Beclin-1	polymer	450	51953.1	1	UniProt (Q14457) Pfam (PF17675) Pfam (PF04111) Pfam (PF15285)	Homo sapiens (human)	Coiled-coil myosin-like BCL2-interacting protein,Protein GT197
5	E (E)	Ras-related protein Rab-1A	polymer	226	25206.5	1	UniProt (P62820) Pfam (PF00071)	Homo sapiens (human)	YPT1-related protein
6	F, I (A, E)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
7	G, J (A, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	H (A)	MYRISTIC ACID	non-polymer		228.4	1	Chemie (MYR)

Sequence modifications

A: 2 - 1358 (UniProt: Q99570)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 1359	-	expression tag
Thr 1360	-	expression tag
Glu 1361	-	expression tag
Asn 1362	-	expression tag
Leu 1363	-	expression tag
Tyr 1364	-	expression tag
Phe 1365	-	expression tag
Gln 1366	-	expression tag
Ser 1367	-	expression tag
Gly 1368	-	expression tag
Met 1369	-	expression tag
Ala 1370	-	expression tag
Ala 1371	-	expression tag
Trp 1372	-	expression tag
Ser 1373	-	expression tag
His 1374	-	expression tag
Pro 1375	-	expression tag
Gln 1376	-	expression tag
Phe 1377	-	expression tag
Glu 1378	-	expression tag
Lys 1379	-	expression tag
Gly 1380	-	expression tag
Gly 1381	-	expression tag
Gly 1382	-	expression tag
Ala 1383	-	expression tag
Arg 1384	-	expression tag
Gly 1385	-	expression tag
Gly 1386	-	expression tag
Ser 1387	-	expression tag
Gly 1388	-	expression tag
Gly 1389	-	expression tag
Gly 1390	-	expression tag
Ser 1391	-	expression tag
Trp 1392	-	expression tag
Ser 1393	-	expression tag
His 1394	-	expression tag
Pro 1395	-	expression tag
Gln 1396	-	expression tag
Phe 1397	-	expression tag
Glu 1398	-	expression tag
Lys 1399	-	expression tag
Gly 1400	-	expression tag
Phe 1401	-	expression tag
Asp 1402	-	expression tag
Tyr 1403	-	expression tag
Lys 1404	-	expression tag
Asp 1405	-	expression tag
Asp 1406	-	expression tag
Asp 1407	-	expression tag
Asp 1408	-	expression tag
Lys 1409	-	expression tag

E: 1 - 205 (UniProt: P62820)

PDB	External Database	Details
Met -20	-	expression tag
Lys -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Ser -4	-	expression tag
Asn -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Leu 70	Gln 70	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	393035.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1323.3
All*	Total formula weight	394359.1

*Water molecules are not included.