9MHF

Cryo-EM reconstruction of PI3KC3-C1 in complex with Human RAB1A(Q70L)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phosphoinositide 3-kinase regulatory subunit 4	polymer	1409	158808.5	1	UniProt (Q99570) Pfam (PF00069) Pfam (PF22956) Pfam (PF00400)	Homo sapiens (human)	PI3-kinase regulatory subunit 4,PI3-kinase p150 subunit,Phosphoinositide 3-kinase adaptor protein
2	B (B)	Phosphatidylinositol 3-kinase catalytic subunit type 3	polymer	887	101680.3	1	UniProt (Q8NEB9) Pfam (PF00792) Pfam (PF00613) Pfam (PF00454)	Homo sapiens (human)	PI3-kinase type 3,PI3K type 3,PtdIns-3-kinase type 3,Phosphatidylinositol 3-kinase p100 subunit,Phosphoinositide-3-kinase class 3,hVps34
3	C (C)	Beclin 1-associated autophagy-related key regulator	polymer	492	55387.3	1	UniProt (Q6ZNE5) Pfam (PF10186)	Homo sapiens (human)
4	D (D)	Beclin-1	polymer	450	51953.1	1	UniProt (Q14457) Pfam (PF17675) Pfam (PF04111) Pfam (PF15285)	Homo sapiens (human)	Coiled-coil myosin-like BCL2-interacting protein,Protein GT197
5	E (E)	Ras-related protein Rab-1A	polymer	226	25206.5	1	UniProt (P62820) Pfam (PF00071)	Homo sapiens (human)	YPT1-related protein
6	F, I (A, E)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
7	G, J (A, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	H (A)	MYRISTIC ACID	non-polymer		228.4	1	Chemie (MYR)
9	K, L (A, E)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A: 2 - 1358 (UniProt: Q99570)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 1359	-	expression tag
Thr 1360	-	expression tag
Glu 1361	-	expression tag
Asn 1362	-	expression tag
Leu 1363	-	expression tag
Tyr 1364	-	expression tag
Phe 1365	-	expression tag
Gln 1366	-	expression tag
Ser 1367	-	expression tag
Gly 1368	-	expression tag
Met 1369	-	expression tag
Ala 1370	-	expression tag
Ala 1371	-	expression tag
Trp 1372	-	expression tag
Ser 1373	-	expression tag
His 1374	-	expression tag
Pro 1375	-	expression tag
Gln 1376	-	expression tag
Phe 1377	-	expression tag
Glu 1378	-	expression tag
Lys 1379	-	expression tag
Gly 1380	-	expression tag
Gly 1381	-	expression tag
Gly 1382	-	expression tag
Ala 1383	-	expression tag
Arg 1384	-	expression tag
Gly 1385	-	expression tag
Gly 1386	-	expression tag
Ser 1387	-	expression tag
Gly 1388	-	expression tag
Gly 1389	-	expression tag
Gly 1390	-	expression tag
Ser 1391	-	expression tag
Trp 1392	-	expression tag
Ser 1393	-	expression tag
His 1394	-	expression tag
Pro 1395	-	expression tag
Gln 1396	-	expression tag
Phe 1397	-	expression tag
Glu 1398	-	expression tag
Lys 1399	-	expression tag
Gly 1400	-	expression tag
Phe 1401	-	expression tag
Asp 1402	-	expression tag
Tyr 1403	-	expression tag
Lys 1404	-	expression tag
Asp 1405	-	expression tag
Asp 1406	-	expression tag
Asp 1407	-	expression tag
Asp 1408	-	expression tag
Lys 1409	-	expression tag

E: 1 - 205 (UniProt: P62820)

PDB	External Database	Details
Met -20	-	expression tag
Lys -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Ser -4	-	expression tag
Asn -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Leu 70	Gln 70	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	393035.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1323.3
All*	Total formula weight	394359.1

*Water molecules are not included.