9M1H
Cryo-EM structure of PGE2-EP1-Gq complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Prostaglandin E2 receptor EP1 subtype | polymer | 402 | 41847.2 | 1 | UniProt (P34995) Pfam (PF00001) | Homo sapiens (human) | PGE receptor EP1 subtype,PGE2 receptor EP1 subtype,Prostanoid EP1 receptor |
| 2 | B (B) | Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(q) subunit alpha | polymer | 361 | 41724.4 | 1 | UniProt (P63096) UniProt (P63092) UniProt (P50148) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein,Guanine nucleotide-binding protein alpha-q |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (A) | (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid | non-polymer | 352.5 | 1 | Chemie (P2E) | |||
| 6 | F (A) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
B: 1 - 19 (UniProt: P63096)
B: 20 - 60 (UniProt: P63092)
B: 61 - 180 (UniProt: P63096)
B: 181 - 228 (UniProt: P63092)
B: 231 - 346 (UniProt: P63092)
B: 347 - 361 (UniProt: P50148)
C: 7 - 345 (UniProt: P62873)
B: 20 - 60 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Arg 32 | Ala 39 | conflict |
| Leu 34 | His 41 | conflict |
| Asp 42 | Gly 49 | conflict |
| Asn 43 | Glu 50 | conflict |
| Tyr 56 | Leu 63 | conflict |
B: 181 - 228 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Ala 203 | Gly 226 | conflict |
| Asp 226 | Ala 249 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asp 229 | - | linker |
| Tyr 230 | - | linker |
| Asp 239 | Leu 272 | conflict |
| Lys 310 | Asp 343 | conflict |
| Val 313 | Leu 346 | conflict |
| Asp 314 | Arg 347 | conflict |
| Ile 325 | Tyr 358 | conflict |
| Ser 333 | Ala 366 | conflict |
| Ala 339 | Ile 372 | conflict |
| Ile 342 | Val 375 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ile 349 | Thr 347 | conflict |
| Met 353 | Leu 351 | conflict |
| Arg 356 | Lys 354 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Gln 6 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 129348.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 352.5 | |
| All* | Total formula weight | 129700.7 |
