9LWF

Cryo-EM structure of dual sensor bound GATOR2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, J, K (A, B, K, L)	GATOR2 complex protein MIOS	polymer	875	98700.4	4	UniProt (Q9NXC5) Pfam (PF21720) Pfam (PF21719) Pfam (PF17034)	Homo sapiens (human)	Missing oocyte meiosis regulator homolog
2	C, I (C, I)	GATOR2 complex protein WDR24	polymer	790	88327.0	2	UniProt (Q96S15) Pfam (PF00400)	Homo sapiens (human)	WD repeat-containing protein 24
3	D, L (D, N)	GATOR2 complex protein WDR59	polymer	974	109938.4	2	UniProt (Q6PJI9) Pfam (PF00400) Pfam (PF17120)	Homo sapiens (human)	WD repeat-containing protein 59
4	E, F, G, M, N... (E, F, G, O, P...)	Isoform B of Nucleoporin SEH1	polymer	421	46636.3	6	UniProt (Q96EE3) Pfam (PF00400)	Homo sapiens (human)	GATOR2 complex protein SEH1,Nup107-160 subcomplex subunit SEH1,SEC13-like protein
5	H, P (H, R)	Isoform 3 of Protein SEC13 homolog	polymer	368	40791.7	2	UniProt (P55735) Pfam (PF00400)	Homo sapiens (human)	GATOR2 complex protein SEC13,SEC13-like protein 1,SEC13-related protein
6	Q, R (U, V)	Cytosolic arginine sensor for mTORC1 subunit 1	polymer	329	36265.2	2	UniProt (Q8WTX7) Pfam (PF18700) Pfam (PF13840) Pfam (PF21389)	Homo sapiens (human)	Cellular arginine sensor for mTORC1 protein 1,GATS-like protein 3
7	S, T (X, Y)	Sestrin-2	polymer	480	54544.6	2	UniProt (P58004) Pfam (PF04636)	Homo sapiens (human)	Hypoxia-induced gene
8	AA, BA, CA, DA, EA... (B, C, D, I, K...)	ZINC ION	non-polymer		65.4	32	Chemie (ZN)

Sequence modifications

U, V: 1 - 329 (UniProt: Q8WTX7)

PDB	External Database	Details
Ala 304	Asp 304	engineered mutation

X, Y: 1 - 480 (UniProt: P58004)

PDB	External Database	Details
Phe 375	Tyr 375	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	1334352.9
Non-Polymers*	Number of molecules	32
	Total formula weight	2093.1
All*	Total formula weight	1336446.0

*Water molecules are not included.