9LGI
Cryo-EM structure of a type II-D CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | HNH nuclease domain-containing protein | polymer | 762 | 88439.4 | 1 | UniProt (A0A1G1G089) | Nitrospirae bacterium RBG_13_39_12 | CRISPR-associated endonuclease Cas9/Csn1 |
| 2 | B (B) | sgRNA (156-MER) | polymer | 156 | 50211.6 | 1 | Nitrospirae bacterium RBG_13_39_12 | ||
| 3 | C (C) | DNA (41-MER) | polymer | 41 | 12489.1 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (41-MER) | polymer | 41 | 12560.1 | 1 | synthetic construct | ||
| 5 | E, F (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 6 | G, H (C, D) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 163700.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 130.8 | |
| All* | Total formula weight | 163831.0 |
