9LGI
Cryo-EM structure of a type II-D CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.86 |
| Software | PHENIX |
| CTF correction | NONE |
