9L6X

Crystal structure of the L7Ae derivative protein LS4 in complex with its co-evolved target CS1 RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Large ribosomal subunit protein eL8	polymer	119	13208.2	2	UniProt (O29494) Pfam (PF01248)	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	50S ribosomal protein L7Ae,Ribosomal protein L8e
2	B, D (B, D)	CS1 RNA	polymer	36	11747.1	2		synthetic construct
3	E, F, G (B)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4	H, I, J, K (A, B, C, D)	water	water		18.0	222	Chemie (HOH)

Sequence modifications

A, C: 2 - 119 (UniProt: O29494)

PDB	External Database	Details
Ser 1	-	expression tag
Arg 34	Glu 34	engineered mutation
His 37	Lys 37	engineered mutation
Tyr 40	Glu 40	engineered mutation
Gly 88	Ile 88	engineered mutation
Trp 89	Glu 89	engineered mutation
Pro 90	Val 90	engineered mutation
Ile 91	Pro 91	engineered mutation
Gly 92	Cys 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	49910.6
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	49983.5

*Water molecules are not included.