9L6X
Crystal structure of the L7Ae derivative protein LS4 in complex with its co-evolved target CS1 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.870, 91.880, 61.210 |
| Unit cell angles | 90.00, 106.11, 90.00 |
Refinement procedure
| Resolution | 45.940 - 1.890 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bw0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmeas | 0.115 | 1.785 |
| Number of reflections | 34786 | 5390 |
| <I/σ(I)> | 11.35 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.999 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4% Tacsimate, pH 4.0, 12% PEG 3,350 |
