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9L5T

Cryo-EM structure of the thermophile spliceosome (state B*Q2)

This is a non-PDB format compatible entry.
Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(2)
U2 snRNApolymer19361393.81Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
2B
(5)
U5 snRNApolymer11636997.81Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
3C
(6)
U6 snRNApolymer10132443.31GenBank (170940748)Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
4D
(A)
PRP8polymer2463285131.01Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
5E
(B)
Pre-mRNA-splicing factor SYF2polymer32636945.91UniProt (G0S5N3)
Pfam (PF08231)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)SYF2
6F
(C)
SNU114polymer1011113867.61Pfam (PF00679)
Pfam (PF16004)
Pfam (PF00009)
Pfam (PF03144)
Pfam (PF03764)
UniProt (by SIFTS) (G0SBV2)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
7G
(D)
SDE2polymer32535780.91UniProt (G0RZD9)
Pfam (PF13019)
Pfam (PF22782)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)SDE2
8H
(E)
Anaphase-promoting complex subunit 4-like WD40 domain-containing proteinpolymer35238230.01UniProt (G0S9U7)
Pfam (PF00400)
Pfam (PF12894)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)U5-40K
9I
(F)
CCDC12polymer23325360.01UniProt (G0SDJ6)
Pfam (PF08315)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)CCDC12
10J
(I)
Putative pre-mRNA splicing proteinpolymer83997944.21UniProt (G0S620)
Pfam (PF05843)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)SYF1
11K
(J)
Suppressor of forked domain-containing proteinpolymer68782195.41UniProt (G0S0H7)
Pfam (PF05843)
Pfam (PF02184)
Pfam (PF13428)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)SYF3
12L
(L)
Putative pre-mRNA splicing proteinpolymer76886636.21UniProt (G0S8A6)
Pfam (PF13921)
Pfam (PF11831)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)CDC5L
13M
(K)
Pre-mRNA-splicing factor SPF27polymer23125550.71UniProt (G0SDM8)
Pfam (PF05700)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)SPF27
14N
(Y)
Pre-mRNA-splicing factorpolymer1416161045.51UniProt (G0SFN9)
Pfam (PF16399)
Pfam (PF21143)
Pfam (PF21144)
Pfam (PF13086)
Pfam (PF13087)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)AQR
15O, P, Q, R
(q, t, r, s)
Pre-mRNA-processing factor 19polymer48051844.34UniProt (G0SFY0)
Pfam (PF08606)
Pfam (PF00400)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)PRP19
16S
(N)
Putative bud site selection proteinpolymer14817176.81UniProt (G0S4Q2)
Pfam (PF01125)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)BUD31
17T
(S)
Peptidyl-prolyl cis-trans isomerasepolymer16718361.81UniProt (G0S1V3)
Pfam (PF00160)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)PPIL1,PPIase
18U
(T)
Pre-mRNA-splicing factor PRP46polymer49654207.31UniProt (G0S066)
Pfam (PF00400)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)PLRG1,Pre-mRNA-processing protein 46
19V
(M)
Putative pre-mRNA splicing proteinpolymer39542241.81UniProt (G0RYD8)
Pfam (PF21369)
Pfam (PF00076)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)ECM2
20W
(0)
Putative pre-mRNA splicing proteinpolymer40845885.21UniProt (G0S7S7)
Pfam (PF16131)
Pfam (PF00076)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)CWC2
21X
(R)
Pre-mRNA-processing protein 45polymer57865034.61UniProt (G0SF87)
Pfam (PF02731)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)SKIP
22Y
(W)
PRP17polymer54761222.01UniProt (G0S2N1)
Pfam (PF00400)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)PRP17
23Z
(P)
Putative pre-mRNA splicing proteinpolymer26028956.61UniProt (G0S754)
Pfam (PF04889)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)CWC15
24AA
(j)
Small nuclear ribonucleoprotein Epolymer9810967.81UniProt (G0S8J0)
Pfam (PF01423)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)smE,snRNP-E,Sm protein E
25BA
(k)
Small nuclear ribonucleoprotein Gpolymer829259.81UniProt (G0SI53)
Pfam (PF01423)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)smG,snRNP-G
26CA
(l)
Sm protein Fpolymer9410727.21UniProt (G0RY82)
Pfam (PF01423)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)smF
27DA
(m)
Delta(14)-sterol reductasepolymer59266785.51UniProt (G0S405)
Pfam (PF01222)
Pfam (PF01423)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)smD2,C-14 sterol reductase,Sterol C14-reductase
28EA
(o)
Small nuclear ribonucleoprotein Sm D1polymer11813033.11UniProt (G0RZL0)
Pfam (PF01423)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)smD1,snRNP core protein D1
29FA
(p)
Sm protein Bpolymer21121425.51UniProt (G0SHQ3)
Pfam (PF01423)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)smB/B'
30GA
(u)
Small nuclear ribonucleoprotein Sm D3polymer11412708.71UniProt (G0SG70)
Pfam (PF01423)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)smD3,Sm-D3,snRNP core protein D3
31HA
(1)
GPATCH1polymer69875861.01Pfam (PF07713)
Pfam (PF01585)
UniProt (by SIFTS) (G0SA63)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
32IA
(z)
RNA helicasepolymer67274989.81UniProt (G0RZD6)
Pfam (PF00270)
Pfam (PF00271)
Pfam (PF04408)
Pfam (PF21010)
Pfam (PF07717)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)DHX35
33JA
(V)
Putative pre-mRNA splicing proteinpolymer22326329.31UniProt (G0S4I7)
Pfam (PF08312)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)SRRM2
34KA
(Z)
Putative pre-mRNA splicing proteinpolymer67876518.11UniProt (G0S5W9)
Pfam (PF02854)
Pfam (PF02847)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)CWC22
35LA
(CY)
Nineteen complex-related protein 2-domain-containing proteinpolymer51056472.41UniProt (G0SI54)
Pfam (PF15458)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
36MA
(Ck)
GCFC2polymer423592.41Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
37NA
(Cb)
TFIP11polymer39133293.91Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
38OA
(8)
Unknown mRNApolymer226293.11Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
39PA
(Cc)
RNA helicasepolymer76487155.51UniProt (G0RY84)
Pfam (PF00270)
Pfam (PF00271)
Pfam (PF04408)
Pfam (PF21010)
Pfam (PF07717)
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)DHX15
40QA
(2)
N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate)non-polymer487.31Chemie (M7M)
41RA
(C)
GUANOSINE-5'-TRIPHOSPHATEnon-polymer523.21Chemie (GTP)
42SA, TA, UA, VA, WA
(N, M)
ZINC IONnon-polymer65.45Chemie (ZN)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains42
Total formula weight2245398.6
Non-Polymers*Number of molecules7
Total formula weight1337.5
All*Total formula weight2246736.2
*Water molecules are not included.

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PDB entries from 2025-07-16

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