9L5T

Cryo-EM structure of the thermophile spliceosome (state B*Q2)

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
0	0000974	cellular_component	Prp19 complex
0	0003723	molecular_function	RNA binding
0	0005634	cellular_component	nucleus
0	0005681	cellular_component	spliceosomal complex
0	0006397	biological_process	mRNA processing
0	0008270	molecular_function	zinc ion binding
0	0008380	biological_process	RNA splicing
0	0017070	molecular_function	U6 snRNA binding
0	0036002	molecular_function	pre-mRNA binding
0	0046872	molecular_function	metal ion binding
0	0071006	cellular_component	U2-type catalytic step 1 spliceosome
0	0071007	cellular_component	U2-type catalytic step 2 spliceosome
B	0000398	biological_process	mRNA splicing, via spliceosome
B	0000974	cellular_component	Prp19 complex
B	0005634	cellular_component	nucleus
B	0005681	cellular_component	spliceosomal complex
B	0006397	biological_process	mRNA processing
B	0008380	biological_process	RNA splicing
B	0071013	cellular_component	catalytic step 2 spliceosome
B	0071014	cellular_component	post-mRNA release spliceosomal complex
Cc	0000166	molecular_function	nucleotide binding
Cc	0000390	biological_process	spliceosomal complex disassembly
Cc	0003723	molecular_function	RNA binding
Cc	0004386	molecular_function	helicase activity
Cc	0005524	molecular_function	ATP binding
Cc	0005634	cellular_component	nucleus
Cc	0005681	cellular_component	spliceosomal complex
Cc	0006397	biological_process	mRNA processing
Cc	0008380	biological_process	RNA splicing
Cc	0016787	molecular_function	hydrolase activity
Cc	0046872	molecular_function	metal ion binding
Cc	0071014	cellular_component	post-mRNA release spliceosomal complex
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0006397	biological_process	mRNA processing
D	0008380	biological_process	RNA splicing
E	0003723	molecular_function	RNA binding
E	0006397	biological_process	mRNA processing
E	0008380	biological_process	RNA splicing
E	0071013	cellular_component	catalytic step 2 spliceosome
F	0005684	cellular_component	U2-type spliceosomal complex
F	0071014	cellular_component	post-mRNA release spliceosomal complex
I	0000349	biological_process	generation of catalytic spliceosome for first transesterification step
I	0000398	biological_process	mRNA splicing, via spliceosome
I	0000974	cellular_component	Prp19 complex
I	0005634	cellular_component	nucleus
I	0005681	cellular_component	spliceosomal complex
I	0006397	biological_process	mRNA processing
I	0008380	biological_process	RNA splicing
I	0071007	cellular_component	U2-type catalytic step 2 spliceosome
I	0071014	cellular_component	post-mRNA release spliceosomal complex
J	0000245	biological_process	spliceosomal complex assembly
J	0000398	biological_process	mRNA splicing, via spliceosome
J	0000974	cellular_component	Prp19 complex
J	0005634	cellular_component	nucleus
J	0005681	cellular_component	spliceosomal complex
J	0006397	biological_process	mRNA processing
J	0008380	biological_process	RNA splicing
J	0071007	cellular_component	U2-type catalytic step 2 spliceosome
J	0071011	cellular_component	precatalytic spliceosome
J	0071014	cellular_component	post-mRNA release spliceosomal complex
K	0000974	cellular_component	Prp19 complex
K	0005634	cellular_component	nucleus
K	0005681	cellular_component	spliceosomal complex
K	0006397	biological_process	mRNA processing
K	0008380	biological_process	RNA splicing
K	0071011	cellular_component	precatalytic spliceosome
K	0071013	cellular_component	catalytic step 2 spliceosome
L	0000398	biological_process	mRNA splicing, via spliceosome
L	0000974	cellular_component	Prp19 complex
L	0003677	molecular_function	DNA binding
L	0005634	cellular_component	nucleus
L	0005681	cellular_component	spliceosomal complex
L	0006397	biological_process	mRNA processing
L	0008380	biological_process	RNA splicing
M	0000974	cellular_component	Prp19 complex
M	0003723	molecular_function	RNA binding
M	0005634	cellular_component	nucleus
M	0005681	cellular_component	spliceosomal complex
M	0006397	biological_process	mRNA processing
M	0008380	biological_process	RNA splicing
M	0017070	molecular_function	U6 snRNA binding
M	0036002	molecular_function	pre-mRNA binding
M	0071006	cellular_component	U2-type catalytic step 1 spliceosome
M	0071007	cellular_component	U2-type catalytic step 2 spliceosome
N	0000398	biological_process	mRNA splicing, via spliceosome
N	0005634	cellular_component	nucleus
N	0005681	cellular_component	spliceosomal complex
P	0003723	molecular_function	RNA binding
P	0006397	biological_process	mRNA processing
P	0008380	biological_process	RNA splicing
P	0045292	biological_process	mRNA cis splicing, via spliceosome
P	0071013	cellular_component	catalytic step 2 spliceosome
R	0005634	cellular_component	nucleus
R	0005681	cellular_component	spliceosomal complex
R	0006397	biological_process	mRNA processing
R	0008380	biological_process	RNA splicing
S	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
S	0005634	cellular_component	nucleus
S	0016853	molecular_function	isomerase activity
S	0071013	cellular_component	catalytic step 2 spliceosome
T	0000398	biological_process	mRNA splicing, via spliceosome
T	0000974	cellular_component	Prp19 complex
T	0005634	cellular_component	nucleus
T	0005681	cellular_component	spliceosomal complex
T	0006397	biological_process	mRNA processing
T	0008380	biological_process	RNA splicing
T	0071011	cellular_component	precatalytic spliceosome
T	0071013	cellular_component	catalytic step 2 spliceosome
V	0005634	cellular_component	nucleus
V	0005681	cellular_component	spliceosomal complex
V	0006397	biological_process	mRNA processing
V	0008380	biological_process	RNA splicing
W	0000398	biological_process	mRNA splicing, via spliceosome
W	0003729	molecular_function	mRNA binding
W	0071013	cellular_component	catalytic step 2 spliceosome
Y	0003729	molecular_function	mRNA binding
Y	0005634	cellular_component	nucleus
Y	0005684	cellular_component	U2-type spliceosomal complex
Y	0006397	biological_process	mRNA processing
Y	0008380	biological_process	RNA splicing
Y	0045292	biological_process	mRNA cis splicing, via spliceosome
Y	0071013	cellular_component	catalytic step 2 spliceosome
Z	0000398	biological_process	mRNA splicing, via spliceosome
Z	0003723	molecular_function	RNA binding
Z	0005634	cellular_component	nucleus
Z	0005681	cellular_component	spliceosomal complex
Z	0006397	biological_process	mRNA processing
Z	0008380	biological_process	RNA splicing
Z	0071013	cellular_component	catalytic step 2 spliceosome
j	0000387	biological_process	spliceosomal snRNP assembly
j	0003723	molecular_function	RNA binding
j	0005634	cellular_component	nucleus
j	0005681	cellular_component	spliceosomal complex
j	0005682	cellular_component	U5 snRNP
j	0005685	cellular_component	U1 snRNP
j	0005686	cellular_component	U2 snRNP
j	0005687	cellular_component	U4 snRNP
j	0005737	cellular_component	cytoplasm
j	0006397	biological_process	mRNA processing
j	0008380	biological_process	RNA splicing
j	0032991	cellular_component	protein-containing complex
j	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
j	1990904	cellular_component	ribonucleoprotein complex
k	0000387	biological_process	spliceosomal snRNP assembly
k	0000398	biological_process	mRNA splicing, via spliceosome
k	0003723	molecular_function	RNA binding
k	0005634	cellular_component	nucleus
k	0005681	cellular_component	spliceosomal complex
k	0005682	cellular_component	U5 snRNP
k	0005685	cellular_component	U1 snRNP
k	0005686	cellular_component	U2 snRNP
k	0005687	cellular_component	U4 snRNP
k	0006397	biological_process	mRNA processing
k	0008380	biological_process	RNA splicing
k	0032991	cellular_component	protein-containing complex
k	0034719	cellular_component	SMN-Sm protein complex
k	0071004	cellular_component	U2-type prespliceosome
k	0071011	cellular_component	precatalytic spliceosome
k	0071013	cellular_component	catalytic step 2 spliceosome
k	0097526	cellular_component	spliceosomal tri-snRNP complex
k	1990904	cellular_component	ribonucleoprotein complex
l	0000398	biological_process	mRNA splicing, via spliceosome
l	0003723	molecular_function	RNA binding
l	0005634	cellular_component	nucleus
l	0005681	cellular_component	spliceosomal complex
l	0005685	cellular_component	U1 snRNP
l	0006397	biological_process	mRNA processing
l	0008380	biological_process	RNA splicing
l	0034715	cellular_component	pICln-Sm protein complex
l	0071013	cellular_component	catalytic step 2 spliceosome
l	0120114	cellular_component	Sm-like protein family complex
l	1990904	cellular_component	ribonucleoprotein complex
m	0000398	biological_process	mRNA splicing, via spliceosome
m	0000974	cellular_component	Prp19 complex
m	0003723	molecular_function	RNA binding
m	0005634	cellular_component	nucleus
m	0005681	cellular_component	spliceosomal complex
m	0005682	cellular_component	U5 snRNP
m	0005685	cellular_component	U1 snRNP
m	0005737	cellular_component	cytoplasm
m	0005789	cellular_component	endoplasmic reticulum membrane
m	0005829	cellular_component	cytosol
m	0006397	biological_process	mRNA processing
m	0006629	biological_process	lipid metabolic process
m	0006694	biological_process	steroid biosynthetic process
m	0006696	biological_process	ergosterol biosynthetic process
m	0008202	biological_process	steroid metabolic process
m	0008380	biological_process	RNA splicing
m	0016020	cellular_component	membrane
m	0016126	biological_process	sterol biosynthetic process
m	0016491	molecular_function	oxidoreductase activity
m	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
m	0050613	molecular_function	Delta14-sterol reductase activity
m	1990904	cellular_component	ribonucleoprotein complex
o	0000387	biological_process	spliceosomal snRNP assembly
o	0003723	molecular_function	RNA binding
o	0005634	cellular_component	nucleus
o	0006397	biological_process	mRNA processing
o	0008380	biological_process	RNA splicing
o	0010468	biological_process	regulation of gene expression
o	0031981	cellular_component	nuclear lumen
o	0097525	cellular_component	spliceosomal snRNP complex
o	1990904	cellular_component	ribonucleoprotein complex
p	0000398	biological_process	mRNA splicing, via spliceosome
p	0003723	molecular_function	RNA binding
p	0005634	cellular_component	nucleus
p	0005682	cellular_component	U5 snRNP
p	0005685	cellular_component	U1 snRNP
p	0005686	cellular_component	U2 snRNP
p	0005687	cellular_component	U4 snRNP
p	0005737	cellular_component	cytoplasm
p	0006397	biological_process	mRNA processing
p	0008380	biological_process	RNA splicing
p	0032991	cellular_component	protein-containing complex
p	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
p	0070990	molecular_function	snRNP binding
p	0071004	cellular_component	U2-type prespliceosome
p	0071013	cellular_component	catalytic step 2 spliceosome
p	1990904	cellular_component	ribonucleoprotein complex
q	0000398	biological_process	mRNA splicing, via spliceosome
q	0000974	cellular_component	Prp19 complex
q	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
q	0004842	molecular_function	ubiquitin-protein transferase activity
q	0005634	cellular_component	nucleus
q	0005681	cellular_component	spliceosomal complex
q	0005737	cellular_component	cytoplasm
q	0006281	biological_process	DNA repair
q	0006397	biological_process	mRNA processing
q	0006974	biological_process	DNA damage response
q	0008380	biological_process	RNA splicing
q	0016567	biological_process	protein ubiquitination
q	0016740	molecular_function	transferase activity
q	0061630	molecular_function	ubiquitin protein ligase activity
q	0070534	biological_process	protein K63-linked ubiquitination
q	0071006	cellular_component	U2-type catalytic step 1 spliceosome
r	0000398	biological_process	mRNA splicing, via spliceosome
r	0000974	cellular_component	Prp19 complex
r	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
r	0004842	molecular_function	ubiquitin-protein transferase activity
r	0005634	cellular_component	nucleus
r	0005681	cellular_component	spliceosomal complex
r	0005737	cellular_component	cytoplasm
r	0006281	biological_process	DNA repair
r	0006397	biological_process	mRNA processing
r	0006974	biological_process	DNA damage response
r	0008380	biological_process	RNA splicing
r	0016567	biological_process	protein ubiquitination
r	0016740	molecular_function	transferase activity
r	0061630	molecular_function	ubiquitin protein ligase activity
r	0070534	biological_process	protein K63-linked ubiquitination
r	0071006	cellular_component	U2-type catalytic step 1 spliceosome
s	0000398	biological_process	mRNA splicing, via spliceosome
s	0000974	cellular_component	Prp19 complex
s	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
s	0004842	molecular_function	ubiquitin-protein transferase activity
s	0005634	cellular_component	nucleus
s	0005681	cellular_component	spliceosomal complex
s	0005737	cellular_component	cytoplasm
s	0006281	biological_process	DNA repair
s	0006397	biological_process	mRNA processing
s	0006974	biological_process	DNA damage response
s	0008380	biological_process	RNA splicing
s	0016567	biological_process	protein ubiquitination
s	0016740	molecular_function	transferase activity
s	0061630	molecular_function	ubiquitin protein ligase activity
s	0070534	biological_process	protein K63-linked ubiquitination
s	0071006	cellular_component	U2-type catalytic step 1 spliceosome
t	0000398	biological_process	mRNA splicing, via spliceosome
t	0000974	cellular_component	Prp19 complex
t	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
t	0004842	molecular_function	ubiquitin-protein transferase activity
t	0005634	cellular_component	nucleus
t	0005681	cellular_component	spliceosomal complex
t	0005737	cellular_component	cytoplasm
t	0006281	biological_process	DNA repair
t	0006397	biological_process	mRNA processing
t	0006974	biological_process	DNA damage response
t	0008380	biological_process	RNA splicing
t	0016567	biological_process	protein ubiquitination
t	0016740	molecular_function	transferase activity
t	0061630	molecular_function	ubiquitin protein ligase activity
t	0070534	biological_process	protein K63-linked ubiquitination
t	0071006	cellular_component	U2-type catalytic step 1 spliceosome
u	0000387	biological_process	spliceosomal snRNP assembly
u	0003723	molecular_function	RNA binding
u	0005634	cellular_component	nucleus
u	0005685	cellular_component	U1 snRNP
u	0005829	cellular_component	cytosol
u	0006397	biological_process	mRNA processing
u	0008380	biological_process	RNA splicing
u	1990904	cellular_component	ribonucleoprotein complex
z	0000166	molecular_function	nucleotide binding
z	0003723	molecular_function	RNA binding
z	0004386	molecular_function	helicase activity
z	0005524	molecular_function	ATP binding
z	0016787	molecular_function	hydrolase activity
z	0071013	cellular_component	catalytic step 2 spliceosome
z	1990904	cellular_component	ribonucleoprotein complex

Functional Information from PROSITE/UniProt

site_id	PS00131
Number of Residues	8
Details	CARBOXYPEPT_SER_SER Serine carboxypeptidases, serine active site. MySESYGG

Chain	Residue	Details
C	MET370-GLY377

---

site_id	PS00170
Number of Residues	18
Details	CSA_PPIASE_1 Cyclophilin-type peptidyl-prolyl cis-trans isomerase signature. YdgTiFHRVIkdFMvQGG

Chain	Residue	Details
S	TYR39-GLY56

---

site_id	PS00430
Number of Residues	63
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. mklvrflmkcanetvtielkngaivhgtiasvtpkmdtalrnvrytpkgeepysv............................................................ESLTVRGN

Chain	Residue	Details
o	MET1-ASN63

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LFSAsaDmHLASWDL

Chain	Residue	Details
E	LEU118-LEU132
E	ILE202-ILE216
T	LEU286-MET300
T	VAL328-LEU342

---

site_id	PS00690
Number of Residues	10
Details	DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. SvIMIDEAHE

Chain	Residue	Details
z	SER137-GLU146
Cc	SER213-GLU222

---

site_id	PS00997
Number of Residues	23
Details	G10_1 G10 protein signature 1. LCClRCiqtkEtNfnstCiCRVP

Chain	Residue	Details
N	LEU102-PRO124

---

site_id	PS01017
Number of Residues	16
Details	STEROL_REDUCT_1 Sterol reductase family signature 1. GniLYDwFiGreLNPR

Chain	Residue	Details
m	GLY-285-ARG-270

---

site_id	PS01018
Number of Residues	24
Details	STEROL_REDUCT_2 Sterol reductase family signature 2. LLLHRdrRDdekCskKYGedWekY

Chain	Residue	Details
m	LEU-32-TYR-9

---