9KTZ

Cryo-EM structure of the TIA-1 prion-like domain amyloid fibril, G355R

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (B, E, A, D, C...)	Cytotoxic granule associated RNA binding protein TIA1	polymer	91	10445.3	6	UniProt (P31483)	Homo sapiens (human)	Nucleolysin TIA-1 isoform p40,RNA-binding protein TIA-1,T-cell-restricted intracellular antigen-1,TIA-1,p40-TIA-1

Sequence modifications

B, E, A, D, C, F: -4 - 62 (UniProt: P31483)

PDB	External Database	Details
Met -28	-	initiating methionine
Gly -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Glu -11	-	expression tag
Asn -10	-	expression tag
Leu -9	-	expression tag
Tyr -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Arg 31	Gly 355	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	62671.6
All*	Total formula weight	62671.6

*Water molecules are not included.