9KTZ
Cryo-EM structure of the TIA-1 prion-like domain amyloid fibril, G355R
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.1 |
| Number of Particle | 610417 |
| Software | RELION,SerialEM,CTFFIND,Coot,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
