9KP6

Cryo-EM structure of mouse bestrophin-1 in a closed state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Bestrophin-1,Soluble cytochrome b562	polymer	689	79285.3	5	UniProt (O88870) UniProt (P0ABE7) Pfam (PF01062) UniProt (by SIFTS) (O76090)	Mus musculus (house mouse)	Vitelliform macular dystrophy protein 2 homolog,Cytochrome b-562
2	F, I, L, O, S (A, B, C, D, E)	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)
3	G, H, J, K, M... (A, B, C, D, E)	CHLORIDE ION	non-polymer		35.5	10	Chemie (CL)

Sequence modifications

A, B, C, D, E: 1 - 551 (UniProt: O88870)

PDB	External Database	Details
Thr 552	-	linker
Gly 553	-	linker
Leu 554	-	linker
Glu 555	-	linker
Val 556	-	linker
Leu 557	-	linker
Phe 558	-	linker
Gln 559	-	linker
Gly 560	-	linker
Pro 561	-	linker

A, B, C, D, E: 562 - 667 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 568	Met 29	engineered mutation
Ile 663	His 124	engineered mutation
Leu 667	Arg 128	engineered mutation
Asp 668	-	expression tag
Tyr 669	-	expression tag
Lys 670	-	expression tag
Asp 671	-	expression tag
His 672	-	expression tag
Asp 673	-	expression tag
Gly 674	-	expression tag
Asp 675	-	expression tag
Tyr 676	-	expression tag
Lys 677	-	expression tag
Asp 678	-	expression tag
His 679	-	expression tag
Asp 680	-	expression tag
Ile 681	-	expression tag
Asp 682	-	expression tag
Tyr 683	-	expression tag
Lys 684	-	expression tag
Asp 685	-	expression tag
Asp 686	-	expression tag
Asp 687	-	expression tag
Asp 688	-	expression tag
Lys 689	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	396426.3
Non-Polymers*	Number of molecules	15
	Total formula weight	554.9
All*	Total formula weight	396981.3

*Water molecules are not included.