9KHS
Cryo-EM structure of Ufd2/Ubc4-Ub in complex with K29-linked diUb (monomeric conformation)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Ubiquitin-conjugating enzyme E2 4 | polymer | 148 | 16442.6 | 1 | UniProt (P15731) Pfam (PF00179) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | E2 ubiquitin-conjugating enzyme 4,Ubiquitin carrier protein 4,Ubiquitin-protein ligase 4 |
| 2 | B (D) | Polyubiquitin-C | polymer | 77 | 8665.9 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (human) | |
| 3 | C (E) | Polyubiquitin-C | polymer | 76 | 8604.8 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (human) | |
| 4 | D (C) | Ubiquitin | polymer | 76 | 8576.8 | 1 | UniProt (P62979) Pfam (PF00240) UniProt (by SIFTS) (P0CG48) | Homo sapiens (human) | |
| 5 | E (A) | E4 ubiquitin-protein ligase UFD2 | polymer | 961 | 110044.5 | 1 | UniProt (P54860) Pfam (PF10408) Pfam (PF04564) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | RING-type E3 ubiquitin transferase UFD2,Ubiquitin conjugation factor E4,Ubiquitin fusion degradation protein 2,UB fusion protein 2 |
Sequence modifications
B: 1 - 148 (UniProt: P15731)
D: 1 - 76 (UniProt: P0CG48)
E: 1 - 76 (UniProt: P0CG48)
| PDB | External Database | Details |
|---|---|---|
| Ser 22 | Cys 22 | engineered mutation |
| Ser 108 | Cys 108 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 48 | Lys 48 | engineered mutation |
| Asp 77 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Arg 29 | Lys 29 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 152334.7 | |
| All* | Total formula weight | 152334.7 |
