9KBZ
Cryo-EM structure of DCL4-DRB4CTD-dsRNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dicer-like protein 4 | polymer | 1720 | 193967.4 | 1 | UniProt (P84634) Pfam (PF00270) Pfam (PF00271) Pfam (PF03368) Pfam (PF00636) Pfam (PF14709) | Arabidopsis thaliana (thale cress) | |
| 2 | B (B) | Double-stranded RNA-binding protein 4 | polymer | 117 | 13526.0 | 1 | UniProt (Q8H1D4) | Arabidopsis thaliana (thale cress) | dsRNA-binding protein 4,AtDRB4 |
| 3 | C (C) | RNA (57-MER) | polymer | 57 | 18249.8 | 1 | GenBank (20197647) | Arabidopsis thaliana | |
| 4 | D (D) | RNA (55-MER) | polymer | 55 | 17610.5 | 1 | GenBank (20197647) | Arabidopsis thaliana | RNA (5'-phosphorylated 55 MER) |
| 5 | E, F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) |
Sequence modifications
A: 62 - 1702 (UniProt: P84634)
B: 292 - 355 (UniProt: Q8H1D4)
| PDB | External Database | Details |
|---|---|---|
| Met 9 | - | initiating methionine |
| Trp 10 | - | expression tag |
| Ser 11 | - | expression tag |
| His 12 | - | expression tag |
| Pro 13 | - | expression tag |
| Gln 14 | - | expression tag |
| Phe 15 | - | expression tag |
| Glu 16 | - | expression tag |
| Lys 17 | - | expression tag |
| Gly 18 | - | expression tag |
| Ser 19 | - | expression tag |
| Gly 20 | - | expression tag |
| Ser 21 | - | expression tag |
| Gly 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Gly 24 | - | expression tag |
| Ser 25 | - | expression tag |
| Trp 26 | - | expression tag |
| Ser 27 | - | expression tag |
| His 28 | - | expression tag |
| Pro 29 | - | expression tag |
| Gln 30 | - | expression tag |
| Phe 31 | - | expression tag |
| Glu 32 | - | expression tag |
| Lys 33 | - | expression tag |
| Ser 34 | - | expression tag |
| Tyr 35 | - | expression tag |
| Tyr 36 | - | expression tag |
| His 37 | - | expression tag |
| His 38 | - | expression tag |
| His 39 | - | expression tag |
| His 40 | - | expression tag |
| His 41 | - | expression tag |
| His 42 | - | expression tag |
| His 43 | - | expression tag |
| His 44 | - | expression tag |
| His 45 | - | expression tag |
| His 46 | - | expression tag |
| Asp 47 | - | expression tag |
| Tyr 48 | - | expression tag |
| Asp 49 | - | expression tag |
| Ile 50 | - | expression tag |
| Pro 51 | - | expression tag |
| Thr 52 | - | expression tag |
| Thr 53 | - | expression tag |
| Glu 54 | - | expression tag |
| Asn 55 | - | expression tag |
| Leu 56 | - | expression tag |
| Tyr 57 | - | expression tag |
| Phe 58 | - | expression tag |
| Gln 59 | - | expression tag |
| Gly 60 | - | expression tag |
| Ala 61 | - | expression tag |
| Asn 1237 | Asp 1237 | conflict |
| Gln 1240 | Glu 1240 | conflict |
| Asn 1422 | Asp 1422 | conflict |
| Gln 1425 | Glu 1425 | conflict |
| Asp 1703 | - | expression tag |
| Tyr 1704 | - | expression tag |
| Lys 1705 | - | expression tag |
| Asp 1706 | - | expression tag |
| Asp 1707 | - | expression tag |
| Asp 1708 | - | expression tag |
| Asp 1709 | - | expression tag |
| Lys 1710 | - | expression tag |
| Gly 1711 | - | expression tag |
| Asp 1712 | - | expression tag |
| Tyr 1713 | - | expression tag |
| Lys 1714 | - | expression tag |
| Asp 1715 | - | expression tag |
| Asp 1716 | - | expression tag |
| Asp 1717 | - | expression tag |
| Asp 1718 | - | expression tag |
| Lys 1719 | - | expression tag |
| Ile 1720 | - | expression tag |
| Asp 1721 | - | expression tag |
| Tyr 1722 | - | expression tag |
| Lys 1723 | - | expression tag |
| Asp 1724 | - | expression tag |
| Asp 1725 | - | expression tag |
| Asp 1726 | - | expression tag |
| Asp 1727 | - | expression tag |
| Lys 1728 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 239 | - | initiating methionine |
| Trp 240 | - | expression tag |
| Ser 241 | - | expression tag |
| His 242 | - | expression tag |
| Pro 243 | - | expression tag |
| Gln 244 | - | expression tag |
| Phe 245 | - | expression tag |
| Glu 246 | - | expression tag |
| Lys 247 | - | expression tag |
| Gly 248 | - | expression tag |
| Ser 249 | - | expression tag |
| Gly 250 | - | expression tag |
| Ser 251 | - | expression tag |
| Gly 252 | - | expression tag |
| Ser 253 | - | expression tag |
| Gly 254 | - | expression tag |
| Ser 255 | - | expression tag |
| Trp 256 | - | expression tag |
| Ser 257 | - | expression tag |
| His 258 | - | expression tag |
| Pro 259 | - | expression tag |
| Gln 260 | - | expression tag |
| Phe 261 | - | expression tag |
| Glu 262 | - | expression tag |
| Lys 263 | - | expression tag |
| Ser 264 | - | expression tag |
| Tyr 265 | - | expression tag |
| Tyr 266 | - | expression tag |
| His 267 | - | expression tag |
| His 268 | - | expression tag |
| His 269 | - | expression tag |
| His 270 | - | expression tag |
| His 271 | - | expression tag |
| His 272 | - | expression tag |
| His 273 | - | expression tag |
| His 274 | - | expression tag |
| His 275 | - | expression tag |
| His 276 | - | expression tag |
| Asp 277 | - | expression tag |
| Tyr 278 | - | expression tag |
| Asp 279 | - | expression tag |
| Ile 280 | - | expression tag |
| Pro 281 | - | expression tag |
| Thr 282 | - | expression tag |
| Thr 283 | - | expression tag |
| Glu 284 | - | expression tag |
| Asn 285 | - | expression tag |
| Leu 286 | - | expression tag |
| Tyr 287 | - | expression tag |
| Phe 288 | - | expression tag |
| Gln 289 | - | expression tag |
| Gly 290 | - | expression tag |
| Ala 291 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 243353.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 72.9 | |
| All* | Total formula weight | 243426.6 |
