9K7M

Coprinopsis cinerea GH131 protein CcGH131B E161A in complex with cellobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glycoside hydrolase 131 catalytic N-terminal domain-containing protein	polymer	313	35161.7	2	UniProt (D6RP27) Pfam (PF18271)	Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus Hormographiella aspergillata)
2	C, D (C, D)	beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900023) GlyTouCan (G86802XL)
3	E, G (A, B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
4	F (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	H (B)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
6	I, J (A, B)	water	water		18.0	911	Chemie (HOH)

Sequence modifications

A, B: 18 - 318 (UniProt: D6RP27)

PDB	External Database	Details
Met 17	-	initiating methionine
Ala 87	Thr 87	engineered mutation
Ala 161	Glu 161	engineered mutation
Glu 247	Lys 247	engineered mutation
Ala 319	-	expression tag
Ala 320	-	expression tag
Ala 321	-	expression tag
Leu 322	-	expression tag
Glu 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70323.3
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	4
	Total formula weight	646.8
All*	Total formula weight	71654.7

*Water molecules are not included.