9K0X
Cryo-EM structure of ATP-bound P2Y purinoceptor 2-miniGq-Nb35 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 243 | 28014.8 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39418.1 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (I) | P2Y purinoceptor 2 | polymer | 420 | 47053.7 | 1 | UniProt (P41231) Pfam (PF00001) | Homo sapiens (human) | P2Y2,ATP receptor,P2U purinoceptor 1,P2U1,P2U receptor 1,Purinergic receptor |
| 5 | E (N) | Nanobody 35 | polymer | 134 | 14686.3 | 1 | Vicugna pacos | ||
| 6 | F (I) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) |
Sequence modifications
A: 26 - 195 (UniProt: P63092)
A: 204 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
I: 1 - 377 (UniProt: P41231)
| PDB | External Database | Details |
|---|---|---|
| Thr 11 | - | expression tag |
| Leu 12 | - | expression tag |
| Ser 13 | - | expression tag |
| Ala 14 | - | expression tag |
| Glu 15 | - | expression tag |
| Asp 16 | - | expression tag |
| Lys 17 | - | expression tag |
| Ala 18 | - | expression tag |
| Ala 19 | - | expression tag |
| Val 20 | - | expression tag |
| Glu 21 | - | expression tag |
| Arg 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Lys 24 | - | expression tag |
| Met 25 | - | expression tag |
| Asp 49 | Gly 49 | conflict |
| Asn 50 | Glu 50 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 196 | - | linker |
| Gly 197 | - | linker |
| Ser 198 | - | linker |
| Gly 199 | - | linker |
| Gly 200 | - | linker |
| Ser 201 | - | linker |
| Gly 202 | - | linker |
| Gly 203 | - | linker |
| Asp 249 | Ala 249 | conflict |
| Asp 252 | Ser 252 | conflict |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Asp 272 | Leu 272 | conflict |
| Ala 372 | Ile 372 | conflict |
| Ile 375 | Val 375 | conflict |
| Lys 380 | Arg 380 | conflict |
| Leu 384 | Gln 384 | conflict |
| Gln 385 | Arg 385 | conflict |
| Asn 387 | His 387 | conflict |
| Glu 390 | Gln 390 | conflict |
| Asn 392 | Glu 392 | conflict |
| Val 394 | Leu 394 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -23 | - | initiating methionine |
| Lys -22 | - | expression tag |
| Thr -21 | - | expression tag |
| Ile -20 | - | expression tag |
| Ile -19 | - | expression tag |
| Ala -18 | - | expression tag |
| Leu -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Tyr -15 | - | expression tag |
| Ile -14 | - | expression tag |
| Phe -13 | - | expression tag |
| Cys -12 | - | expression tag |
| Leu -11 | - | expression tag |
| Val -10 | - | expression tag |
| Phe -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Leu 46 | Pro 46 | variant |
| Asn 302 | Asp 302 | engineered mutation |
| Ser 312 | Arg 312 | variant |
| His 378 | - | expression tag |
| His 379 | - | expression tag |
| His 380 | - | expression tag |
| His 381 | - | expression tag |
| His 382 | - | expression tag |
| His 383 | - | expression tag |
| Gly 384 | - | expression tag |
| Gly 385 | - | expression tag |
| Ser 386 | - | expression tag |
| Gly 387 | - | expression tag |
| Gly 388 | - | expression tag |
| Leu 389 | - | expression tag |
| Glu 390 | - | expression tag |
| Val 391 | - | expression tag |
| Leu 392 | - | expression tag |
| Phe 393 | - | expression tag |
| Gln 394 | - | expression tag |
| Gly 395 | - | expression tag |
| Pro 396 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 137034.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 507.2 | |
| All* | Total formula weight | 137541.2 |
