9JQP

Cryo-EM structure of the HMBPP-primed BTN3A1-BTN3A2-BTN2A1 in complex with agonist antibody TH001

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Butyrophilin subfamily 3 member A1	polymer	527	58247.7	1	UniProt (O00481) Pfam (PF07686) Pfam (PF22705) Pfam (PF13765) Pfam (PF00622)	Homo sapiens (human)
2	B (B)	Butyrophilin subfamily 3 member A2	polymer	318	34629.9	1	UniProt (P78410) Pfam (PF07686) Pfam (PF22705)	Homo sapiens (human)
3	C (E)	Butyrophilin subfamily 2 member A1	polymer	532	60015.3	1	UniProt (Q7KYR7) Pfam (PF00622) Pfam (PF07686) Pfam (PF22705) Pfam (PF13765)	Homo sapiens (human)
4	D, F (H, J)	BTN3A agonist antibody TH001, variable domain of heavy chain	polymer	117	12819.2	2		Mus musculus
5	E, G (I, K)	BTN3A agonist antibody TH001, variable domain of light chain	polymer	112	12253.8	2		Mus musculus
6	H, I, J, K, L... (A, B, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A: 1 - 484 (UniProt: O00481)

PDB	External Database	Details
Gly 485	-	expression tag
Gly 486	-	expression tag
Gly 487	-	expression tag
Gly 488	-	expression tag
Ser 489	-	expression tag
Gly 490	-	expression tag
Gly 491	-	expression tag
Gly 492	-	expression tag
Gly 493	-	expression tag
Ser 494	-	expression tag
Gly 495	-	expression tag
Gly 496	-	expression tag
Gly 497	-	expression tag
Gly 498	-	expression tag
Ser 499	-	expression tag
Trp 500	-	expression tag
Ser 501	-	expression tag
His 502	-	expression tag
Pro 503	-	expression tag
Gln 504	-	expression tag
Phe 505	-	expression tag
Glu 506	-	expression tag
Lys 507	-	expression tag
Gly 508	-	expression tag
Gly 509	-	expression tag
Gly 510	-	expression tag
Ser 511	-	expression tag
Gly 512	-	expression tag
Gly 513	-	expression tag
Gly 514	-	expression tag
Ser 515	-	expression tag
Gly 516	-	expression tag
Gly 517	-	expression tag
Ser 518	-	expression tag
Ala 519	-	expression tag
Trp 520	-	expression tag
Ser 521	-	expression tag
His 522	-	expression tag
Pro 523	-	expression tag
Gln 524	-	expression tag
Phe 525	-	expression tag
Glu 526	-	expression tag
Lys 527	-	expression tag

B: 1 - 305 (UniProt: P78410)

PDB	External Database	Details
Gly 306	-	expression tag
Gly 307	-	expression tag
Gly 308	-	expression tag
Gly 309	-	expression tag
Ser 310	-	expression tag
Asp 311	-	expression tag
Tyr 312	-	expression tag
Lys 313	-	expression tag
Asp 314	-	expression tag
Asp 315	-	expression tag
Asp 316	-	expression tag
Asp 317	-	expression tag
Lys 318	-	expression tag

E: 1 - 499 (UniProt: Q7KYR7)

PDB	External Database	Details
Gly 500	-	expression tag
Gly 501	-	expression tag
Gly 502	-	expression tag
Gly 503	-	expression tag
Ser 504	-	expression tag
Trp 505	-	expression tag
Ser 506	-	expression tag
His 507	-	expression tag
Pro 508	-	expression tag
Gln 509	-	expression tag
Phe 510	-	expression tag
Glu 511	-	expression tag
Lys 512	-	expression tag
Gly 513	-	expression tag
Gly 514	-	expression tag
Gly 515	-	expression tag
Ser 516	-	expression tag
Gly 517	-	expression tag
Gly 518	-	expression tag
Gly 519	-	expression tag
Ser 520	-	expression tag
Gly 521	-	expression tag
Gly 522	-	expression tag
Ser 523	-	expression tag
Ala 524	-	expression tag
Trp 525	-	expression tag
Ser 526	-	expression tag
His 527	-	expression tag
Pro 528	-	expression tag
Gln 529	-	expression tag
Phe 530	-	expression tag
Glu 531	-	expression tag
Lys 532	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	203038.9
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	204366.1

*Water molecules are not included.