9JKQ

Cryo-EM structure of the METH-bound hTAAR1-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nb35	polymer	162	17540.7	1		Homo sapiens
5	E (R)	Soluble cytochrome b562,Trace amine-associated receptor 1	polymer	510	57969.6	1	UniProt (P0ABE7) UniProt (Q96RJ0) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,TaR-1,Trace amine receptor 1
6	F (R)	(2S)-N-methyl-1-phenylpropan-2-amine	non-polymer		149.2	1	Chemie (B40)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	conflict
Ala 226	Gly 226	conflict
Ala 268	Glu 268	conflict
Lys 271	Asn 271	conflict
Asp 274	Lys 274	conflict
Lys 280	Arg 280	conflict
Asp 284	Thr 284	conflict
Thr 285	Ile 285	conflict

B: 13 - 351 (UniProt: P62873)

PDB	External Database	Details
Met 1	-	initiating methionine
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Leu 10	-	expression tag
Leu 11	-	expression tag
Gln 12	-	expression tag

C: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	conflict

R: -105 - 0 (UniProt: P0ABE7)

PDB	External Database	Details
Met -124	-	initiating methionine
Lys -123	-	expression tag
Thr -122	-	expression tag
Ile -121	-	expression tag
Ile -120	-	expression tag
Ala -119	-	expression tag
Leu -118	-	expression tag
Ser -117	-	expression tag
Tyr -116	-	expression tag
Ile -115	-	expression tag
Phe -114	-	expression tag
Cys -113	-	expression tag
Leu -112	-	expression tag
Val -111	-	expression tag
Phe -110	-	expression tag
Ala -109	-	expression tag
Gly -108	-	expression tag
Ala -107	-	expression tag
Pro -106	-	expression tag
Trp -99	Met 29	conflict
Ile -4	His 124	conflict
Leu 0	Arg 128	conflict

R: 1 - 339 (UniProt: Q96RJ0)

PDB	External Database	Details
Val 63	His 63	conflict
Trp 112	Phe 112	conflict
Glu 340	-	expression tag
Phe 341	-	expression tag
Leu 342	-	expression tag
Glu 343	-	expression tag
Val 344	-	expression tag
Leu 345	-	expression tag
Phe 346	-	expression tag
Gln 347	-	expression tag
Gly 348	-	expression tag
Pro 349	-	expression tag
Trp 350	-	expression tag
Ser 351	-	expression tag
His 352	-	expression tag
Pro 353	-	expression tag
Gln 354	-	expression tag
Phe 355	-	expression tag
Glu 356	-	expression tag
Lys 357	-	expression tag
Gly 358	-	expression tag
Gly 359	-	expression tag
Gly 360	-	expression tag
Ser 361	-	expression tag
Gly 362	-	expression tag
Gly 363	-	expression tag
Gly 364	-	expression tag
Ser 365	-	expression tag
Gly 366	-	expression tag
Gly 367	-	expression tag
Ser 368	-	expression tag
Ala 369	-	expression tag
Trp 370	-	expression tag
Ser 371	-	expression tag
His 372	-	expression tag
Pro 373	-	expression tag
Gln 374	-	expression tag
Phe 375	-	expression tag
Glu 376	-	expression tag
Lys 377	-	expression tag
Asp 378	-	expression tag
Tyr 379	-	expression tag
Lys 380	-	expression tag
Asp 381	-	expression tag
Asp 382	-	expression tag
Asp 383	-	expression tag
Asp 384	-	expression tag
Lys 385	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	167783.3
Non-Polymers*	Number of molecules	1
	Total formula weight	149.2
All*	Total formula weight	167932.5

*Water molecules are not included.