9JKB

Cryo-EM structure of the CUL1-RBX1-SKP1-FBXO4 SCF ubiquition ligase complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (C, D)	F-box only protein 4	polymer	315	36247.1	2	UniProt (Q9UKT5) Pfam (PF00646)	Homo sapiens (human)
2	B (A)	Cullin-1	polymer	796	91715.4	1	UniProt (Q13616) Pfam (PF00888) Pfam (PF10557)	Homo sapiens (human)	CUL-1
3	C (B)	S-phase kinase-associated protein 1	polymer	166	18881.1	1	UniProt (P63208) Pfam (PF03931) Pfam (PF01466)	Homo sapiens (human)	Cyclin-A/CDK2-associated protein p19,p19A,Organ of Corti protein 2,OCP-2,Organ of Corti protein II,OCP-II,RNA polymerase II elongation factor-like protein,SIII,Transcription elongation factor B polypeptide 1-like,p19skp1
4	D (R)	E3 ubiquitin-protein ligase RBX1, N-terminally processed	polymer	96	11196.8	1	UniProt (P62877) Pfam (PF12678)	Homo sapiens (human)	E3 ubiquitin-protein transferase RBX1,N-terminally processed

Sequence modifications

C, D: 75 - 387 (UniProt: Q9UKT5)

PDB	External Database	Details
His 73	-	expression tag
Met 74	-	expression tag
-	Thr 150	deletion
-	Arg 151	deletion
-	Arg 152	deletion
-	Ala 153	deletion
-	Ser 154	deletion
-	Lys 155	deletion
-	Ser 156	deletion
-	Ser 157	deletion
-	Arg 158	deletion
-	Pro 159	deletion
-	Met 160	deletion
-	Tyr 161	deletion
-	Gly 162	deletion
-	Ala 163	deletion
-	Val 164	deletion
-	Thr 165	deletion
-	Ser 166	deletion
-	Phe 167	deletion
-	Leu 168	deletion
-	His 169	deletion
-	Ser 170	deletion

A: 2 - 776 (UniProt: Q13616)

PDB	External Database	Details
Ser -19	-	expression tag
Ala -18	-	expression tag
Ser -17	-	expression tag
Trp -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Pro -13	-	expression tag
Gln -12	-	expression tag
Phe -11	-	expression tag
Glu -10	-	expression tag
Lys -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Lys 0	-	expression tag
Leu 1	-	expression tag

B: 1 - 163 (UniProt: P63208)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

R: 16 - 108 (UniProt: P62877)

PDB	External Database	Details
Ser 13	-	expression tag
His 14	-	expression tag
Met 15	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	194287.6
All*	Total formula weight	194287.6

*Water molecules are not included.