9J64
Cryo-EM structure of neddylated Cul2-Rbx1-EloBC-FEM1B homodimer complexed with FNIP1 degron
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, D) | Cullin-2 | polymer | 751 | 87927.8 | 2 | UniProt (Q13617) Pfam (PF00888) Pfam (PF26557) Pfam (PF10557) | Homo sapiens (human) | CUL-2 |
| 2 | C, F (E, H) | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12290.0 | 2 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 3 | D (F) | Elongin-C | polymer | 98 | 11045.7 | 1 | UniProt (Q15369) Pfam (PF03931) | Homo sapiens (human) | EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1 |
| 4 | E (G) | Elongin-B | polymer | 104 | 11748.4 | 1 | UniProt (Q15370) Pfam (PF00240) | Homo sapiens (human) | EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2 |
| 5 | G (I) | Protein fem-1 homolog B | polymer | 628 | 70412.1 | 1 | UniProt (Q9UK73) Pfam (PF12796) | Homo sapiens (human) | FEM1b,FEM1-beta,Fem-1-like death receptor-binding protein alpha,Fem-1-like in apoptotic pathway protein alpha,F1A-alpha |
| 6 | H (J) | Maltose/maltodextrin-binding periplasmic protein,Folliculin interacting protein 1 | polymer | 467 | 51985.3 | 1 | UniProt (P0AEX9) UniProt (J3KNG8) Pfam (PF14637) | Escherichia coli K-12 | MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP |
| 7 | I, J, K, L, M... (E, H, I) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) |
Sequence modifications
A, D: 1 - 745 (UniProt: Q13617)
F: 16 - 112 (UniProt: Q15369)
I: 1 - 627 (UniProt: Q9UK73)
J: 183 - 548 (UniProt: P0AEX9)
J: 563 - 641 (UniProt: J3KNG8)
| PDB | External Database | Details |
|---|---|---|
| His 746 | - | expression tag |
| His 747 | - | expression tag |
| His 748 | - | expression tag |
| His 749 | - | expression tag |
| His 750 | - | expression tag |
| His 751 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 15 | - | initiating methionine |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 175 | - | initiating methionine |
| Gly 176 | - | expression tag |
| His 177 | - | expression tag |
| His 178 | - | expression tag |
| His 179 | - | expression tag |
| His 180 | - | expression tag |
| His 181 | - | expression tag |
| His 182 | - | expression tag |
| Asp 549 | - | linker |
| Tyr 550 | - | linker |
| Asp 551 | - | linker |
| Ile 552 | - | linker |
| Pro 553 | - | linker |
| Thr 554 | - | linker |
| Thr 555 | - | linker |
| Glu 556 | - | linker |
| Asn 557 | - | linker |
| Leu 558 | - | linker |
| Tyr 559 | - | linker |
| Phe 560 | - | linker |
| Gln 561 | - | linker |
| Gly 562 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 345627.1 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 457.9 | |
| All* | Total formula weight | 346085.0 |
