9J4A

Crystal structure of glucose bound highly glucose tolerant GH1 beta-glcosidase mutant (UnBGl1_C188V_H261W)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-glucosidase	polymer	477	52883.7	1	UniProt (K4I4U1)	soil metagenome
2	B (A)	beta-D-glucopyranose	non-polymer		180.2	1	Chemie (BGC)
3	C (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	D (A)	water	water		18.0	338	Chemie (HOH)

Sequence modifications

A: 1 - 469 (UniProt: K4I4U1)

PDB	External Database	Details
Val 188	Cys 188	engineered mutation
Trp 261	His 261	engineered mutation
Leu 470	-	expression tag
Glu 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	52883.7
Non-Polymers*	Number of molecules	2
	Total formula weight	275.1
All*	Total formula weight	53158.8

*Water molecules are not included.