9J0R

Structure of pcStar in the green fluorescent state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green to red photoconvertible GFP-like protein EosFP	polymer	230	26168.7	2	UniProt (Q5S6Z9) Pfam (PF01353)	Lobophyllia hemprichii
2	C (A)	2,3-DIHYDROXY-1,4-DITHIOBUTANE	non-polymer		154.3	1	Chemie (DTT)
3	D, E (A, B)	water	water		18.0	466	Chemie (HOH)

Sequence modifications

A, B: 1 - 226 (UniProt: Q5S6Z9)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Lys 11	Asn 11	engineered mutation
Glu 28	Asp 28	engineered mutation
5sq 64	His 62	chromophore
5sq 64	Tyr 63	chromophore
5sq 64	Gly 64	chromophore
Lys 70	Glu 70	engineered mutation
Asn 74	His 74	engineered mutation
Met 93	Leu 93	engineered mutation
Asn 102	Ile 102	engineered mutation
Tyr 121	His 121	engineered mutation
Thr 123	Val 123	engineered mutation
Glu 158	Thr 158	engineered mutation
Gly 166	Asn 166	engineered mutation
Ala 189	Tyr 189	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52337.4
Non-Polymers*	Number of molecules	1
	Total formula weight	154.3
All*	Total formula weight	52491.6

*Water molecules are not included.