9J0R
Structure of pcStar in the green fluorescent state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.846, 76.768, 124.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.150 - 1.660 |
| R-factor | 0.1618 |
| Rwork | 0.161 |
| R-free | 0.18960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ooz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.478 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.300 | 1.750 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 58118 | 8391 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.6 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.998 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate, pH 4.78 27% (w/v) PEG 4000 |
