9J0O
Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC1-nuc)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit | polymer | 1970 | 217450.1 | 1 | UniProt (A0A8D1DPV6) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) | Sus scrofa (pig) | |
| 2 | B (B) | DNA-directed RNA polymerase subunit beta | polymer | 1174 | 134041.4 | 1 | UniProt (A0A4X1TVZ5) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (A0A0B8RVL1) | Sus scrofa (pig) | |
| 3 | C (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 271 | 30997.6 | 1 | UniProt (A0A481DF93) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (I3LCH3) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit C |
| 4 | D (D) | DNA-directed RNA polymerase II subunit RPB4 | polymer | 142 | 16331.3 | 1 | UniProt (A0A481BYI6) Pfam (PF03874) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit RPB4 isoform X1, DNA-directed RNA polymerase II subunit D |
| 5 | E (E) | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (A0A4X1VTX4) Pfam (PF03871) Pfam (PF01191) UniProt (by SIFTS) (I3LSI7) | Sus scrofa (pig) | RPB5 homolog |
| 6 | F (F) | DNA-directed RNA polymerase II subunit F | polymer | 127 | 14477.0 | 1 | UniProt (A0A4X1VEK9) Pfam (PF01192) | Sus scrofa (pig) | DNA-directed RNA polymerases I,II,and III subunit RPABC2,RPB6 homolog |
| 7 | G (G) | DNA-directed RNA polymerase II subunit RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit G |
| 8 | H (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) Pfam (PF03870) | Sus scrofa (pig) | |
| 9 | I (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) | Sus scrofa (pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 10 | J (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A493TD97) Pfam (PF01194) UniProt (by SIFTS) (A0A8W4F9W9) | Sus scrofa (pig) | |
| 11 | K (K) | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 117 | 13310.3 | 1 | UniProt (F1RKE4) Pfam (PF13656) | Sus scrofa (pig) | RNA POLYMERASE II SUBUNIT J |
| 12 | L (L) | RPB12 | polymer | 58 | 7018.2 | 1 | UniProt (A0A4X1TRS6) Pfam (PF03604) | Sus scrofa (pig) | RNA POLYMERASE II SUBUNIT K |
| 13 | M (Y) | Transcription elongation factor SPT4 | polymer | 118 | 13297.3 | 1 | UniProt (P63272) Pfam (PF06093) | Homo sapiens (human) | hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit |
| 14 | N (Z) | Transcription elongation factor SPT5 | polymer | 1107 | 123297.8 | 1 | UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
| 15 | O (N) | NON-TEMPLATE DNA (180-MER) | polymer | 198 | 61381.9 | 1 | Homo sapiens | ||
| 16 | P (P) | RNA (5'-R(P*AP*AP*UP*UP*AP*GP*CP*UP*CP*UP*UP*GP*UP*GP*UP*U)-3') | polymer | 16 | 5038.0 | 1 | Homo sapiens | ||
| 17 | Q (T) | TEMPLATE DNA (191-MER) | polymer | 198 | 60870.0 | 1 | Homo sapiens | ||
| 18 | R, U (a, e) | Histone H3.3 | polymer | 136 | 15361.0 | 2 | UniProt (P84243) Pfam (PF00125) | Homo sapiens (human) | |
| 19 | S, W (b, f) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62805) Pfam (PF15511) | Homo sapiens (human) | |
| 20 | T, X (c, g) | Histone H2A type 1-B/E | polymer | 130 | 14165.6 | 2 | UniProt (P04908) Pfam (PF00125) Pfam (PF16211) | Homo sapiens (human) | Histone H2A.2,Histone H2A/a,Histone H2A/m |
| 21 | V, Y (d, h) | Histone H2B type 1-J | polymer | 126 | 13935.2 | 2 | UniProt (P06899) Pfam (PF00125) | Homo sapiens (human) | Histone H2B.1,Histone H2B.r,H2B/r |
| 22 | Z (U) | Negative elongation factor A | polymer | 528 | 57343.6 | 1 | UniProt (Q9H3P2) | Homo sapiens (human) | NELF-A,Wolf-Hirschhorn syndrome candidate 2 protein |
| 23 | AA (W) | Negative elongation factor C/D | polymer | 611 | 68547.8 | 1 | UniProt (Q8IXH7) Pfam (PF04858) | Homo sapiens (human) | NELF-C/D,TH1-like protein |
| 24 | BA (V) | Negative elongation factor B | polymer | 580 | 65779.2 | 1 | UniProt (Q8WX92) Pfam (PF06209) | Homo sapiens (human) | NELF-B,Cofactor of BRCA1 |
| 25 | CA (X) | Negative elongation factor E | polymer | 404 | 45852.6 | 1 | UniProt (P18615) Pfam (PF00076) | Homo sapiens (human) | NELF-E,RNA-binding protein RD |
| 26 | DA, EA, GA, HA, IA... (A, B, C, I, J...) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 27 | FA (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
Y: 1 - 117 (UniProt: P63272)
Z: 1 - 1087 (UniProt: O00267)
W: 1 - 590 (UniProt: Q8IXH7)
X: 1 - 380 (UniProt: P18615)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Lys 1088 | - | expression tag |
| Glu 1089 | - | expression tag |
| Thr 1090 | - | expression tag |
| Ala 1091 | - | expression tag |
| Ala 1092 | - | expression tag |
| Ala 1093 | - | expression tag |
| Lys 1094 | - | expression tag |
| Phe 1095 | - | expression tag |
| Glu 1096 | - | expression tag |
| Arg 1097 | - | expression tag |
| Gln 1098 | - | expression tag |
| His 1099 | - | expression tag |
| Met 1100 | - | expression tag |
| Asp 1101 | - | expression tag |
| Ser 1102 | - | expression tag |
| Ser 1103 | - | expression tag |
| Thr 1104 | - | expression tag |
| Ser 1105 | - | expression tag |
| Ala 1106 | - | expression tag |
| Ala 1107 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 591 | - | expression tag |
| Ser 592 | - | expression tag |
| Ser 593 | - | expression tag |
| Gly 594 | - | expression tag |
| Ser 595 | - | expression tag |
| Ser 596 | - | expression tag |
| Gly 597 | - | expression tag |
| Leu 598 | - | expression tag |
| Glu 599 | - | expression tag |
| Val 600 | - | expression tag |
| Leu 601 | - | expression tag |
| Phe 602 | - | expression tag |
| Gln 603 | - | expression tag |
| Gly 604 | - | expression tag |
| Pro 605 | - | expression tag |
| His 606 | - | expression tag |
| His 607 | - | expression tag |
| His 608 | - | expression tag |
| His 609 | - | expression tag |
| His 610 | - | expression tag |
| His 611 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -23 | - | initiating methionine |
| Asp -22 | - | expression tag |
| Tyr -21 | - | expression tag |
| Lys -20 | - | expression tag |
| Asp -19 | - | expression tag |
| Asp -18 | - | expression tag |
| Asp -17 | - | expression tag |
| Asp -16 | - | expression tag |
| Lys -15 | - | expression tag |
| Leu -14 | - | expression tag |
| Glu -13 | - | expression tag |
| Val -12 | - | expression tag |
| Leu -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Gln -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Pro -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Ser -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 29 |
| Total formula weight | 1128063.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 613.0 | |
| All* | Total formula weight | 1128676.6 |
