9IVO
Crystal structure of KRED mutant-Y199A/N149L
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Short-chain dehydrogenase/reductase SDR | polymer | 259 | 26736.7 | 4 | UniProt (A4XEP2) Pfam (PF13561) | Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199) | |
| 2 | E, H, J, M (A, B, C, D) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 4 | Chemie (NAD) | |||
| 3 | F, K (A, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 4 | G, I, L (A, B, D) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 3 | Chemie (DMS) | |||
| 5 | N, O, P, Q (A, B, C, D) | water | water | 18.0 | 372 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 259 (UniProt: A4XEP2)
| PDB | External Database | Details |
|---|---|---|
| Leu 149 | Asn 149 | engineered mutation |
| Ala 199 | Tyr 199 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 106947.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 3012.2 | |
| All* | Total formula weight | 109959.2 |
